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Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu

Year 2023, Volume: 25 Issue: 74, 263 - 274, 15.05.2023

Doruk İlmenöz Zalike Keskin Elif Esin Hameş Tuna

https://doi.org/10.21205/deufmd.2023257401

Abstract

Bakteriyel selüloz (BS), biyouyumlu, üretimi kolay, yüksek çekme dayanımı gösteren, nanofibril ağ yapısı ile yüksek su tutma özelliğinde olan ve bazı mikroorganizmalar tarafından üretilebilen doğal bir polimerdir. Medikal uygulamalar için iyi bir aday olan BS, membran yapısı nedeniyle deri ikamesi çalışmalarında geliştirilebilir bir potansiyel taşımaktadır. Ancak, sıkı yapıdaki selüloz nanofibrilleri hücre tutunması ve göçüne imkân vermemektedir. Bu çalışma kapsamında deri ikamesi olarak kullanılabilecek yeterli gözenek çapına sahip BS’nin in situ üretimi, keratin ile modifikasyonu ve karakterizasyonu amaçlanmıştır.
Gluconacetobacter xylinus ATCC 700178 suşu kullanılarak iki farklı yöntemle (agar parçalama ve agar damlatma) selüloz nanofibrilleri arasındaki gözenek çapı arttırılarak üretilen BS daha sonra derinin önemli bir bileşeni olan keratin ile modifiye edilmiştir. Keratin kaynağı olarak insan saçları kullanılmış ve Shindai özütlemesi ile keratin elde edilmiştir. Keratin çözeltisi BS membranlara emdirilerek malzemenin karakterizasyonu FTIR (Fourier dönüşümlü kızıl ötesi spektrometresi), SEM (Taramalı elektron mikroskobu) ve mekanik çekme dayanımı testleri ile gerçekleştirilmiştir. Sonuç olarak, arttırılmış gözenek çapına sahip (>100μm) yapısında keratin bulunduran ve 0,1- 0,15 MPa aralığında maksimum çekme dayanımı gösteren, deri ikame adayı olabilecek BS üretimi gerçekleştirilmiştir.

Keywords

Bakteriyel selüloz keratin deri biyokompozit malzemeler

Supporting Institution

Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

FYL-2018-20231

Thanks

Bu çalışma Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından FYL-2018-20231nolu proje ile desteklenmiştir.

References

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Production and characterization of porous bacterial cellulose for skin substitution

Year 2023, Volume: 25 Issue: 74, 263 - 274, 15.05.2023

Doruk İlmenöz Zalike Keskin Elif Esin Hameş Tuna

https://doi.org/10.21205/deufmd.2023257401

Abstract

Bacterial cellulose (BC), is a natural polymer produced by certain microorganisms, which is biocompatible, easy to manufacture, has high tensile strength, and has high water retention with its nanofibril network structure. BC is a good candidate for medical applications, and also has potential in skin substitution studies thanks to its membrane structure. However, its tight cellulose nanofibers do not allow cell attachment and migration. In this study, in situ keratin modified production, and characterization of BC with sufficient pore diameters that can be potential skin substitute was aimed.
BC has been produced by Gluconacetobacter xylinus ATCC 700178 strain using two different methods (agar shredding and agar dropping) to increase the pore diameter between cellulose nanofibrils using and modified with keratin as an important component of the skin. Human hair was used as the source of keratin and keratin was obtained according to the Shindai extraction method. Keratin solution was impregnated with BC membranes and the characterization of the material was carried out by FTIR (Fourier Transform Infrared Spectrometry), SEM (Scanning Electron Microscope) and physical strength tests. As a result, the production of skin replacement candidate BC, containing keratin in its structure with sufficient pore diameter (>100μm) with a tensile strength of the range between 0.1- 0.15 MPa, was produced successfully.

Keywords

Bacterial cellulose keratin skin biocomposite materials

Project Number

FYL-2018-20231

References

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  • [3] Beele, H. 2002. Artificial skin: past, present and future, The International Journal of Artificial Organs, Cilt. 5(3), s. 163-173. DOI: 10.1177/039139880202500302
  • [4] Nyame, T.T., Chiang, H.A., Leavitt, T., Ozambela, M., Orgill, D.P. 2015. Tissue-Engineered Skin Substitutes, Plastic and Reconstructive Surgery, Cilt. 136(6), s. 1379–1388. DOI: 10.1097/PRS.0000000000001748
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  • [6] Emre Oz, Y., Keskin-Erdogan, Z., Safa, N., Hames Tuna, E.E. 2021. A review of functionalised bacterial cellulose for targeted biomedical fields, Journal of Biomaterials Applications. DOI: 10.1177/0885328221998033
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  • [19] Ghalia, M.A., Dahman, Y. 2017. Fabrication and enhanced mechanical properties of porous PLA/PEG copolymer reinforced with bacterial cellulose nanofibers for soft tissue engineering applications, Polymer Testing, Cilt. 61, s. 114-131. DOI: 10.1016/j.polymertesting.2017.05.016
  • [20] Li, Y., Jiang, H., Zheng, W., Gong, N., Chen, L., Jiang, X., Yang, G. 2015. Bacterial cellulose–hyaluronan nanocomposite biomaterials as wound dressings for severe skin injury repair, Journal of Materials Chemistry B, Cilt. 3(17), s. 3498-3507. DOI: 10.1039/C4TB01819B
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  • [22] Huang, L., Chen, X., Nguyen, T.X., Tang, H., Zhang, L., Yang, G. 2013. Nano-cellulose 3D-networks as controlled-release drug carriers. Journal of Materials Chemistry B, Cilt. 1(23), s. 2976-2984. DOI: 10.1039/C3TB20149J
  • [23] Amin, M.C.I.M., Ahmad, N., Halib, N., & Ahmad, I. 2012. Synthesis and characterization of thermo-and pH-responsive bacterial cellulose/acrylic acid hydrogels for drug delivery, Carbohydrate Polymers, Cilt. 88(2), s. 465-473. DOI: 10.1016/j.carbpol.2011.12.022
  • [24] Potivara, K., & Phisalaphong, M. (2019). Development and Characterization of Bacterial Cellulose Reinforced with Natural Rubber. Materials (Basel, Switzerland),12(14), 2323. https://doi.org/10.3390/ma12142323.
  • [25] Si-Qian Chen, Patricia Lopez-Sanchez, Dongjie Wang, Deirdre Mikkelsen, Michael J. Gidley, Mechanical properties of bacterial cellulose synthesised by diverse strains of the genus Komagataeibacter, Food Hydrocolloids, Volume 81, 2018, 87-95, https://doi.org/10.1016/j.foodhyd.2018.02.031
  • [26] Czaja, W., Krystynowicz, A., Bielecki, S., Brown Jr, R.M. 2006. Microbial cellulose—the natural power to heal wounds, Biomaterials, Cilt. 27(2), s. 145-151. DOI: 10.1016/j.biomaterials.2005.07.035
  • [27] Portal, O., Clark, W.A., Levinson, D.J. 2009. Microbial cellulose wound dressing in the treatment of nonhealing lower extremity ulcers, Wounds : A Compendium of Clinical Research and Practice, Cilt. 21(1), s. 1–3. PMID: 25904579
  • [28] Hestrin, S., Schramm, M.J.B.J. 1954. Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose, Biochemical Journal, Cilt. 58(2), s. 345. DOI: 10.1042/bj0580345
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  • [30] Keskin, Z., Sendemir Urkmez, A., Hames, E. E. 2017. Novel keratin modified bacterial cellulose nanocomposite production and characterization for skin tissue engineering, Materials Science and Engineering C, Cilt. 75, s. 1144-1153. DOI: 10.1016/j.msec.2017.03.035
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  • [32] Keskin Z. 2015 Keratin içeren bakteriyel selüloz tabanlı kompozit biyomalze üretimi, karaterizasyon ve biyoyapay deri geliştirilmesinde kullanım potansiyelinin araştırılması. Ege Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 81 s, İzmir.
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There are 47 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Doruk İlmenöz This is me 0000-0002-6850-2934

Zalike Keskin 0000-0001-6789-6954

Elif Esin Hameş Tuna 0000-0001-7302-4781

Project Number FYL-2018-20231
Early Pub Date May 12, 2023
Publication Date May 15, 2023
Published in Issue Year 2023 Volume: 25 Issue: 74

Cite

APA İlmenöz, D., Keskin, Z., & Hameş Tuna, E. E. (2023). Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi, 25(74), 263-274. https://doi.org/10.21205/deufmd.2023257401
AMA İlmenöz D, Keskin Z, Hameş Tuna EE. Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu. DEUFMD. May 2023;25(74):263-274. doi:10.21205/deufmd.2023257401
Chicago İlmenöz, Doruk, Zalike Keskin, and Elif Esin Hameş Tuna. “Deri Ikamesi için gözenekli Bakteriyel selüloz üretimi Ve Karakterizasyonu”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi 25, no. 74 (May 2023): 263-74. https://doi.org/10.21205/deufmd.2023257401.
EndNote İlmenöz D, Keskin Z, Hameş Tuna EE (May 1, 2023) Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi 25 74 263–274.
IEEE D. İlmenöz, Z. Keskin, and E. E. Hameş Tuna, “Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu”, DEUFMD, vol. 25, no. 74, pp. 263–274, 2023, doi: 10.21205/deufmd.2023257401.
ISNAD İlmenöz, Doruk et al. “Deri Ikamesi için gözenekli Bakteriyel selüloz üretimi Ve Karakterizasyonu”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi 25/74 (May 2023), 263-274. https://doi.org/10.21205/deufmd.2023257401.
JAMA İlmenöz D, Keskin Z, Hameş Tuna EE. Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu. DEUFMD. 2023;25:263–274.
MLA İlmenöz, Doruk et al. “Deri Ikamesi için gözenekli Bakteriyel selüloz üretimi Ve Karakterizasyonu”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi, vol. 25, no. 74, 2023, pp. 263-74, doi:10.21205/deufmd.2023257401.
Vancouver İlmenöz D, Keskin Z, Hameş Tuna EE. Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu. DEUFMD. 2023;25(74):263-74.

Dokuz Eylül Üniversitesi, Mühendislik Fakültesi Dekanlığı Tınaztepe Yerleşkesi, Adatepe Mah. Doğuş Cad. No: 207-I / 35390 Buca-İZMİR.