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Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose

Year 2021, Volume: 9 Issue: 4, 1382 - 1394, 31.07.2021
https://doi.org/10.29130/dubited.903157

Abstract

The purpose of the study to increase the conversion ratio of lactose hydrolysis to glucose and galactose via packed bed reactors containing immobilised enzyme and design a reactor that will achieve a high conversion ratio in continuous systems. For this purpose, the dynamic properties of a packed bed reactor containing immobilised β-galactosidase enzyme on Duolite A 568 resin were determined by a stimulus-response technique and moment analysis. The relation between kinetic behaviour and The dynamic response was determined via Peclet number (Pe) and Damkohler number (Da). This study was completed with the development of a mathematical model using parameters such as flow dynamics, column geometry, enzyme immobilization performance and the behaviour of the enzyme in the column. According to the results, the particle size, L/D ratio and flow rate appropriate for this system were determined as 390 m, 10 and 4 mL/min, respectively. Furthermore, 55% conversion of lactose from milk was obtained using the enzyme immobilized packed bed reactor.

Supporting Institution

CUBAP

Project Number

M-343

References

  • [1] M. Messia, T. Candigliota, E. Marconi, “Assessment of quality and technological characterization of lactose-hydrolyzed milk,” Food Chemistry, vol. 104, no.3, pp. 910-917, 2007.
  • [2] E. Jurado, F. Camacho, G. Luzon, J. Vicaria, “A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis,” Enzyme and Microbial Technology, vol. 31, no.3, pp. 300-309, 2002.
  • [3] R. Panesar, P.S. Panesar, R.S. Singh, M.B. Bera, “Applicability Of Alginate Entrapped Yeast Cells For The Production Of Lactose‐Hydrolyzed Milk,” Journal of Food Process Engineering, vol.30, no.4, pp. 472-484, 2007.
  • [4] E. Sharp, N.M. D'Cunha, C. S. Ranadheera, T. Vasiljevic, D.B. Panagiotakos, N. Naumovski, “Effects of lactose-free and low-lactose dairy on symptoms of gastrointestinal health: A systematic,” International Dairy Journal, vol.114, 104936, 2021.
  • [5] D.G. Hatzinikolaou, E. Katsifas, D. Mamma, A.D. Karagouni, P. Christakopoulos, D. Kekos, “Modeling of the simultaneous hydrolysis–ultrafiltration of whey permeate by a thermostable β-galactosidase from Aspergillus niger,” Biochemical Engineering Journal, vol.24, no. 2, pp.161-172, 2005.
  • [6] M. Ladero, A. Santos, F. Garcia-Ochoa, “Diffusion and chemical reaction rates with nonuniform enzyme distribution: An experimental approach,” Biotechnology and Bioengineering, vol.72, no.4, pp.458-467, 2001.
  • [7] I. Roy, M.N. Gupta, “Lactose hydrolysis by Lactozym™ immobilized on cellulose beads in batch and fluidized bed modes,” Process Biochemistry, vol.39, no.3, pp. 325-332, 2003.
  • [8] S. Gürdaş, H.A. Güleç, M. Mutlu, “Immobilization of Aspergillus oryzae β-galactosidase onto Duolite A568 resin via simple adsorption mechanism,” Food and Bioprocess Technology, vol.5, no.3, pp. 904-911, 2012.
  • [9] D. Nemec, J. Levec, “Flow through packed bed reactors: 1. Single-phase flow,” Chemical Engineering Science, vol.60, no.24, pp. 6947-6957, 2005.
  • [10] D. S. Wentworth, D. Skonberg, D.W. Donahue, A. Ghanem, “Application of chitosan‐entrapped β‐galactosidase in a packed‐bed reactor system,” Journal of Applied Polymer Science, vol.91, no.2, pp. 1294-1299, 2004.
  • [11] V.V. Kafarov, “Cybernetic methods in chemistry & Chemical engineering”, Moscow, Mir Publishers, 1976.
  • [12] O. Levenspiel, “Chemical reaction engineering,” Industrial & Engineering Chemistry Research, vol.38, no.11, pp. 4140-4143, 1999.
  • [13] Mutlu, M., Sağ, Y., Kutsal, T., “The adsorption of copper (II) by Z. ramigera immobilized on Ca-alginate in packed bed columns: a dynamic approach by stimulus-response technique and evaluation of adsorption data by moment analysis,” The Chemical Engineering Journal and The Biochemical Engineering Journal, vol.65, no.1, pp.81-86, 1997.
  • [14] J.M. Smith, “Chemical engineering kinetics”, 2nd ed., USA, McGraw-Hill Inc, 1981.
  • [15] I.H. Boyaci, “Dolgulu Kolon Entegre Edilmiş Enzim Elektrot Sistemleri ile Gıda Örneklerinde Glukoz, Sukroz ve Laktoz Miktarının Saptanması,” Doktora tezi, Gıda Mühendisliği Bölümü, Hacettepe Üniversitesi, Ankara, Türkiye, 2001.
  • [16] E.E. Petersen, “Chemical reaction analysis”, Prentice Hall, 1965.
  • [17] M. Mutlu, E. Piskin, “Blood plasma proteins on polyurethane and alkylsiloxane plasma-treated polyurethane surfaces. Dynamic approach by stimulus-response technique”, Medical and Biological Engineering and Computing, vol.28, no.3, pp. 232-236, 1990.
  • [18] S. Gürdas, H.A. Güleç, M. Mutlu, “Adsorption Isotherm and Kinetic Modelling of [beta]-Galactosidase Immobilization onto a Basic Resin (Duolite A568),” Asian Journal of Chemistry vol.23, no.3, pp.1049, 2011
  • [19] Y. Cho, J. Bailey, “Immobilization of enzymes on activated carbon: properties of immobilized glucoamylase, glucose oxidase, and gluconolactonase,” Biotechnology and Bioengineering, vol.20, no.10, pp. 1651-1665, 1978.
  • [20] Q.Z. Zhou, X.D. Chen, “Immobilization of β-galactosidase on graphite surface by glutaraldehyde,” Journal of Food Engineering vol.48, no.1, pp. 69-74, 2001.
  • [21] X. Li, Q.Z. Zhou, X.D. Chen, “Pilot-scale lactose hydrolysis using β-galactosidase immobilized on cotton fabric,” Chemical Engineering and Processing: Process Intensification, vol.46, no.5, pp.497-500, 2007.

Laktozun Enzimatik Hidrolizi için Dolgulu Kolon Reaktör Parametrelerinin Optimizasyonu

Year 2021, Volume: 9 Issue: 4, 1382 - 1394, 31.07.2021
https://doi.org/10.29130/dubited.903157

Abstract

Çalışmanın amacı, enzim tutuklu dolgulu kolon reaktör ile laktoz hidrolizinin glikoz ve galaktoza dönüşüm oranını arttımak ve sürekli sistemde yüksek dönüşüm sağlayacak bir reaktör tasarlamaktır. Bu amaçla Duolite A 568 reçinesine tutuklu β-galaktosidaz enzimi içeren dolgulu kolon reaktörün dinamik davranışı moment analizi ve uyarı cevap tekniği ile belirlendi. Kinetik davranış ile dinamik tepki arasındaki ilişki Peclet sayısı (Pe) ve Damkohler sayısı (Da) ile belirlendi. Bu çalışma akış dinamiği, kolon geometrisi, enzim immobilizasyon performansı ve enzimin kolondaki davranışı gibi parametreler kullanılarak matematiksel bir modelin geliştirilmesi ile tamamlandı. Bu veriler doğrultusunda hazırlanan enzim tutuklu dolgulu kolon reaktör sisteminde kullanılması uygun olan partikül büyüklüğü, kolon L/D oranı ve akış hızı sırasıyla 390 μm, 10 ve 4 mL/dk olarak belirlenmiştir. Ayrıca enzim tutuklu dolgulu kolon reaktör ile sütteki laktozdan 55 % dönüşüm elde edilmiştir.

Project Number

M-343

References

  • [1] M. Messia, T. Candigliota, E. Marconi, “Assessment of quality and technological characterization of lactose-hydrolyzed milk,” Food Chemistry, vol. 104, no.3, pp. 910-917, 2007.
  • [2] E. Jurado, F. Camacho, G. Luzon, J. Vicaria, “A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis,” Enzyme and Microbial Technology, vol. 31, no.3, pp. 300-309, 2002.
  • [3] R. Panesar, P.S. Panesar, R.S. Singh, M.B. Bera, “Applicability Of Alginate Entrapped Yeast Cells For The Production Of Lactose‐Hydrolyzed Milk,” Journal of Food Process Engineering, vol.30, no.4, pp. 472-484, 2007.
  • [4] E. Sharp, N.M. D'Cunha, C. S. Ranadheera, T. Vasiljevic, D.B. Panagiotakos, N. Naumovski, “Effects of lactose-free and low-lactose dairy on symptoms of gastrointestinal health: A systematic,” International Dairy Journal, vol.114, 104936, 2021.
  • [5] D.G. Hatzinikolaou, E. Katsifas, D. Mamma, A.D. Karagouni, P. Christakopoulos, D. Kekos, “Modeling of the simultaneous hydrolysis–ultrafiltration of whey permeate by a thermostable β-galactosidase from Aspergillus niger,” Biochemical Engineering Journal, vol.24, no. 2, pp.161-172, 2005.
  • [6] M. Ladero, A. Santos, F. Garcia-Ochoa, “Diffusion and chemical reaction rates with nonuniform enzyme distribution: An experimental approach,” Biotechnology and Bioengineering, vol.72, no.4, pp.458-467, 2001.
  • [7] I. Roy, M.N. Gupta, “Lactose hydrolysis by Lactozym™ immobilized on cellulose beads in batch and fluidized bed modes,” Process Biochemistry, vol.39, no.3, pp. 325-332, 2003.
  • [8] S. Gürdaş, H.A. Güleç, M. Mutlu, “Immobilization of Aspergillus oryzae β-galactosidase onto Duolite A568 resin via simple adsorption mechanism,” Food and Bioprocess Technology, vol.5, no.3, pp. 904-911, 2012.
  • [9] D. Nemec, J. Levec, “Flow through packed bed reactors: 1. Single-phase flow,” Chemical Engineering Science, vol.60, no.24, pp. 6947-6957, 2005.
  • [10] D. S. Wentworth, D. Skonberg, D.W. Donahue, A. Ghanem, “Application of chitosan‐entrapped β‐galactosidase in a packed‐bed reactor system,” Journal of Applied Polymer Science, vol.91, no.2, pp. 1294-1299, 2004.
  • [11] V.V. Kafarov, “Cybernetic methods in chemistry & Chemical engineering”, Moscow, Mir Publishers, 1976.
  • [12] O. Levenspiel, “Chemical reaction engineering,” Industrial & Engineering Chemistry Research, vol.38, no.11, pp. 4140-4143, 1999.
  • [13] Mutlu, M., Sağ, Y., Kutsal, T., “The adsorption of copper (II) by Z. ramigera immobilized on Ca-alginate in packed bed columns: a dynamic approach by stimulus-response technique and evaluation of adsorption data by moment analysis,” The Chemical Engineering Journal and The Biochemical Engineering Journal, vol.65, no.1, pp.81-86, 1997.
  • [14] J.M. Smith, “Chemical engineering kinetics”, 2nd ed., USA, McGraw-Hill Inc, 1981.
  • [15] I.H. Boyaci, “Dolgulu Kolon Entegre Edilmiş Enzim Elektrot Sistemleri ile Gıda Örneklerinde Glukoz, Sukroz ve Laktoz Miktarının Saptanması,” Doktora tezi, Gıda Mühendisliği Bölümü, Hacettepe Üniversitesi, Ankara, Türkiye, 2001.
  • [16] E.E. Petersen, “Chemical reaction analysis”, Prentice Hall, 1965.
  • [17] M. Mutlu, E. Piskin, “Blood plasma proteins on polyurethane and alkylsiloxane plasma-treated polyurethane surfaces. Dynamic approach by stimulus-response technique”, Medical and Biological Engineering and Computing, vol.28, no.3, pp. 232-236, 1990.
  • [18] S. Gürdas, H.A. Güleç, M. Mutlu, “Adsorption Isotherm and Kinetic Modelling of [beta]-Galactosidase Immobilization onto a Basic Resin (Duolite A568),” Asian Journal of Chemistry vol.23, no.3, pp.1049, 2011
  • [19] Y. Cho, J. Bailey, “Immobilization of enzymes on activated carbon: properties of immobilized glucoamylase, glucose oxidase, and gluconolactonase,” Biotechnology and Bioengineering, vol.20, no.10, pp. 1651-1665, 1978.
  • [20] Q.Z. Zhou, X.D. Chen, “Immobilization of β-galactosidase on graphite surface by glutaraldehyde,” Journal of Food Engineering vol.48, no.1, pp. 69-74, 2001.
  • [21] X. Li, Q.Z. Zhou, X.D. Chen, “Pilot-scale lactose hydrolysis using β-galactosidase immobilized on cotton fabric,” Chemical Engineering and Processing: Process Intensification, vol.46, no.5, pp.497-500, 2007.
There are 21 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Sevim Mazlum 0000-0002-1166-422X

Prof.dr İsmail Boyacı 0000-0003-1333-060X

Mehmet Mutlu 0000-0001-7146-1937

Project Number M-343
Publication Date July 31, 2021
Published in Issue Year 2021 Volume: 9 Issue: 4

Cite

APA Mazlum, S., Boyacı, P. İ., & Mutlu, M. (2021). Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 9(4), 1382-1394. https://doi.org/10.29130/dubited.903157
AMA Mazlum S, Boyacı Pİ, Mutlu M. Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose. DUBİTED. July 2021;9(4):1382-1394. doi:10.29130/dubited.903157
Chicago Mazlum, Sevim, Prof.dr İsmail Boyacı, and Mehmet Mutlu. “Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 9, no. 4 (July 2021): 1382-94. https://doi.org/10.29130/dubited.903157.
EndNote Mazlum S, Boyacı Pİ, Mutlu M (July 1, 2021) Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9 4 1382–1394.
IEEE S. Mazlum, P. İ. Boyacı, and M. Mutlu, “Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose”, DUBİTED, vol. 9, no. 4, pp. 1382–1394, 2021, doi: 10.29130/dubited.903157.
ISNAD Mazlum, Sevim et al. “Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9/4 (July 2021), 1382-1394. https://doi.org/10.29130/dubited.903157.
JAMA Mazlum S, Boyacı Pİ, Mutlu M. Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose. DUBİTED. 2021;9:1382–1394.
MLA Mazlum, Sevim et al. “Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, vol. 9, no. 4, 2021, pp. 1382-94, doi:10.29130/dubited.903157.
Vancouver Mazlum S, Boyacı Pİ, Mutlu M. Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose. DUBİTED. 2021;9(4):1382-94.