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Effects of iloprost infusion on the eyes due to ovarian ischemia reperfusion ınjury: an experimental study in rats

Year 2022, Volume: 1 Issue: 3, 152 - 159, 23.12.2022

Abstract

Background: In this study, it is aimed to investigate the effect of iloprost treatment on the eye, which is a prostaglandin analogue, given to rats after the ovarian ischemia-reperfusion model and to contribute to the literature.
Materials and Methods: A total of 32 female Sprague Dawley rats were used for the experiment. Rats were divided into four groups of eight rat search: control group, ischemia group (I), ischemia-reperfusion (I-R) group, I-R/I. Except for the control group, torsion procedure was performed on all rats. 3-hour torsion in the ischemia group, 3-hour torsion and 3-hour detorsion in the I-R group, 3 hour torsion, 3 hour detorsion and 60 mn intravenous iloprost infusion in the I-R/I. After the procedures in each group, the eye tissues were taken for histopathological examination.
Results: In the histopathological examination of the corneal layer, many large gaps and (decreased collagen cross-linking) were detected between the stromal collagen fiber bundles in group I, I-R and I-R/I unlike the control group. In the histopathological examination of the retinal layer and stroma of the iris, nohemoragicarea and inflammation findings were observed in the examinations performed in all groups, but vascular dilatation was found in groups I, I-R and I-R/I when compared to the control group. A higher increase in vascular dilatation was detected in group I-R/I compared to the other groups.
Conclusions: After I/R, although histopathological changes in the eye tissue such as morphological disorders in the stromal collagen fiber bundles of the corneal layer and vascular dilatation in the stroma of iris are supported by the literature, the protective effect of iloprost on tissue damage has not been fully determined.

References

  • Azarkish F., Armin F., Parvar A.A.A. & Dehghani A. (2021). The influence of renal ischemia-reperfusion injury on remote organs: The histological brain changes in male and female rats. Brain Circ, 7(3), 194-200. doi: 10.4103/bc.bc3_21.
  • Banda M.A., Lefer D.J. & Granger D.N. (1997). Post ischemic endothelium-dependent vascular reactivity is preserved in adhesion molecule deficient mice. Am J Physiol, 273:2721-5. doi: 10.1152/ajpheart.1997.273.6.H2721
  • Birukova A.A., Wu T., Tian Y., Meliton A., Sarich N., TianX. ,Leff A. & Birukov K.G. (2013). Iloprost improves endothelia lbarrier function in lipopolysaccharide-induced lung injury. Eur Respir J, 41(1), 165-176. doi: 10.1183/09031936.00148311.
  • Canacankatan N., Sucu N., Aytacoglu B., Gul O.E., Gorur A., Korkmaz B., Sahan-Firat S., Antmen E.S., Tamer L., Ayaz L., Vezir O., Kanik A. & Tunctan B. (2012). Affirmative effects of iloprost on apoptosis during ischemia-reperfusion injury in kidney as a distant organ. Ren Fail, 34(1), 111-118. doi: 10.3109/0886022X.2011.633446.
  • Carden D.L. & Granger D.N. (2000). Pathophysiology of ischaemia-reperfusion injury. J Pathol, 190(3), 255-66. doi: 10.1002/(SICI)10969896(200002)190:3<255::AID-PATH526>3.0.CO;2-6.
  • Ekim H., Erdoğan H.B., Kutay V., Başel H., Özen S., Hazar A, Akbayrak H. & Özbek H. (2005). Effect of ıschemia/reperfusion ınjury to the lungs caused by cross camping of the abdominal aorta. Van Tıp Dergisi, 12 (3),175-178.
  • Eltzschig H.K. & Collard C.D. (2004). Vascular ischaemia and reperfusion injury. Br Med Bull, 70, 71-86. doi:10.1093/bmb/ldh025
  • Erer D., Özer A., Demirtaş H., Gönül İ., Kara H., Arpacı H., Comu F.M., Oktar G.L., Arslan M. & Küçük A. (2016). Effects of alprostadil and iloprost on renal, lung, and skeletal muscle injury following hindlimb ischemia-reperfusion injury in rats. Drug Des Devel Ther, 19(10), 2651-2658. doi: 10.2147/DDDT.S110529.
  • Erre G.L. & Passiu G. (2009). Antioxidant effect of Iloprost: current knowledge and therapeutic implications for systemic sclerosis. Reumatismo, 61(2), 90-97. doi: 10.4081/reumatismo.2009.90.
  • Fileta J.B., Huang W., Kwon G.P., Filippopoulos T., Ben Y., Dobberfuhl A. & Grosskreutz C.L. (2008). Efficient estimation of retinal ganglion cell number: a stereological approach. Journal of neuroscience methods, 170(1), 1-8.
  • Holger K., Eltzschig H.K. & Eckle T. (2011). Ischemia and reperfusion—from mechanism to translation. NatMed, 17(11), 1391-1401. doi: 10.1038/nm.2507.
  • Huang C., Hong M.K. & Ding D.C. (2017). A review of ovary torsion. CiJiYiXueZaZhi, 29(3), 143-147. doi: 10.4103/tcmj.tcmj_55_17
  • Huang S.S., Wei F.C. & Hung L.M. (2006).Ischemic preconditioning attenuates postischemic leukocyte – endothelial cell interactions role of nitricoxide and protein kinase C. Circ J, 70:1070-1075.
  • Iriz E., Iriz A., Take G., Ozgul H., Oktar L., Demirtas H., Helvacıoğlu F. & Arslan M. (2015). Iloprostand vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits. Bratisl Lek Listy, 116(10), 627-631. doi: 10.4149/bll_2015_121.
  • Karaman S., Ozkan B., Yazir Y., Yardimoglu M., Gok M., Kara O., Vural C., Rencber S., Salih K. & Emek S.K. (2016). Comparison of hyperbaric oxygen versus iloprost treatment in an experimental rat central retinal artery occlusion model. Graefes Arch Clin Exp Ophthalmol, 254(11), 2209-2215. doi: 10.1007/s00417-016-3444-5.
  • Kart C., Aran T., Guven S., Karahan S.C. & Yulug E. (2011). Acute increase in plasma D-dimerlevel in ovarian torsion: an experimental study. Hum Reprod, 26(3), 564-568. doi: 10.1093/humrep/deq378.
  • McNamara D.B., Champion H.C. & Kadowitz PJ, (1998). Nonoperative management of lower extremity arterial disease. Part I. Pharmocologic management of peripheral vascular disease. Surg Clin North Am, 78, 447-456.
  • Nahar, N., Mohamed, S., Mustapha, N.M., Lau, S., Ishak, N.I.M. & Umran, N.S. (2021). Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemicrat model. Naunyn-Schmiedeberg'sArchives of Pharmacology, 394(3), 457-467.
  • Özat M., Gungor T., Barun S., Demirogullari B., Sokmensuer L.K., Gulbahar O., Gursoy D & Muftuoglu S. (2009). The effects of iloprost, a prostacyclin analo-gue, in experimental ischaemia/ reperfusion injury in rat ovaries. Experimental and Toxicologic Pathology, 61(5), 519-527.doi: 10.1016/j.etp.2009.06.007.
  • Özdemir G. & İnanc F. (2005). Zinc may protect remoteocular injury caused by intestinal ischemia reperfusion in rats. Tohoku J ExpMed, 206(3), 247-251. doi: 10.1620/tjem.206.247.
  • Rowlands T.E., Gough M.J. & Homer-Vanniasinkam S. (1999). Do prostaglandins have a salutary role in skeletal muscle ischaemia-reperfusion injury? Eur J Vasc Endovasc Surg, 18(5), 439-444. doi: 10.1053/ejvs.1999.0929.
  • Uludağ Ö. (2019). History and Importance of Ethical Rules in Animal Experiment Studies. Adıyaman Üniversitesi Sağlık Bilimleri Derg. 5(1), 1401–1413.
  • Thomson I.A., Egginton S., Slimms M.H. & Hudlicka O. (1996). Effect of muscle ischemia and iloprost during femoro distal reconstruction on capillary endothelial swelling. Int J Microcirc Clin Exp, 16, 284-290.
  • Wu M.Y. Yiang G.T., Liao W.T., Tsai A.P., Cheng Y.L., Cheng P.W., Li C.Y. & Li C.J. (2018). Current mechanistic concepts in ischemia and reperfusion injury. Cell Physiol Biochem, 46, 1650–1667. doi: 10.1159/000489241.
  • Yellon D.M. & Hausenloy D.J. (2007). Myocardial reperfusion injury. N Engl J Med, 357(11), 1121-1135. doi: 10 .1056/NEJMra071667
  • Yılmaz Y. & Tumkaya L. (2019). Effects of hyperbaric oxygen and iloprost on intestina lischemia-reperfusion induced acute lung injury. Ann Surg Treat Res, 96(1), 34-40. doi: 10.4174/astr.2019.96.1.34.
  • Yu H., Kalogeris T. & Korthuis R. (2019). Reactive species-induced microvascular dysfunction in ischemia/reperfusion. Free Radic Biol Med, 135,182-197. doi: 10.1016/j.freeradbiomed.2019.02.031.
  • Zhou M., Yu Y., Luo X., Wang J., Lan X., Liu P., Feng Y. & Jian W. (2021). Myocardial Ischemia-Reperfusion Injury: Therapeutics from a Mitochondria-Centric Perspective. Cardiology, 146(6), 781-792. doi: 10.1159/000518879.

Over iskemi reperfüzyon hasarına karşı verilen iloprost infüzyonunun göz üzerine etkileri: ratlarda deneysel çalışma

Year 2022, Volume: 1 Issue: 3, 152 - 159, 23.12.2022

Abstract

Amaç: Bu çalışmada, ratlara ovarial iskemi reperfüzyon (İ/R) modeli sonrası verilen prostoglandin analoğu olan iloprost tedavisinin uzak organ olarak göz üzerine etkilerini araştırmak ve literatüre katkıda bulunmak amaçlanmıştır.
Materyal ve Metot: Deney için toplam 32 dişi Sprague Dawley cinsi rat kullanıldı. Ratlar, her birinde sekiz adet olacak şekilde dört gruba ayrıldı; kontrol (K), iskemi (İ), iskemi-reperfüzyon (İ-R) ve iskemi-reperfüzyon/iloprost (İ-R/İ). Kontrol grubu hariç diğer ratlara torsiyon işlemi yapıldı. İskemi grubunda 3 saatlik torsiyon, İ-R grubuna 3 saat torsiyon ve 3 saat detorsiyon, İ-R/İ grubuna 3 saat torsiyon, 3 saat detorsiyon ve 60 dk intravenöz iloprost infüzyonu yapıldı. Her grupta işlemlerden sonra göz dokuları histopatolojik olarak incelenmek üzere alındı.
Bulgular: Kornea tabakasına ait histopatolojik incelemede Grup İ, İ-R ve İ-R/İ’ te kontrol grubundan farklı olarak stromal kollajen lif demetleri arasında çok sayıda geniş boşluklara sebep olan çapraz bağlantılarda azalma tespit edildi. Retina tabakasına ait histopatolojik incelemede tüm gruplarda yapılan incelemeler sonucunda herhangi bir hemarojik alan ve enflamasyon bulgusu gözlenmezken kontrol grubuna kıyaslandığında Grup İ, İ-R ve İ-R/İ’ te iris stromasında vasküler dilatasyon bulgusuna rastlandı. Grup İ-R/İ’te vasküler dilatasyon etkisinde diğer gruplara göre biraz daha artış tespit edildi.
Sonuç: İ/R sonrasında göz dokusunda kornea tabakasına ait stromal kollajen lif demetlerinde çapraz bağlantıda bozukluklar ve iris stroma dokusundaki vasküler dilatasyon gibi histopatolojik değişikliklerin gözlenmesi literatürle desteklenir nitelikte olmakla birlikte iloprostun doku hasarı üzerindeki koruyucu etkisi tam anlamıyla tespit edilemedi.

References

  • Azarkish F., Armin F., Parvar A.A.A. & Dehghani A. (2021). The influence of renal ischemia-reperfusion injury on remote organs: The histological brain changes in male and female rats. Brain Circ, 7(3), 194-200. doi: 10.4103/bc.bc3_21.
  • Banda M.A., Lefer D.J. & Granger D.N. (1997). Post ischemic endothelium-dependent vascular reactivity is preserved in adhesion molecule deficient mice. Am J Physiol, 273:2721-5. doi: 10.1152/ajpheart.1997.273.6.H2721
  • Birukova A.A., Wu T., Tian Y., Meliton A., Sarich N., TianX. ,Leff A. & Birukov K.G. (2013). Iloprost improves endothelia lbarrier function in lipopolysaccharide-induced lung injury. Eur Respir J, 41(1), 165-176. doi: 10.1183/09031936.00148311.
  • Canacankatan N., Sucu N., Aytacoglu B., Gul O.E., Gorur A., Korkmaz B., Sahan-Firat S., Antmen E.S., Tamer L., Ayaz L., Vezir O., Kanik A. & Tunctan B. (2012). Affirmative effects of iloprost on apoptosis during ischemia-reperfusion injury in kidney as a distant organ. Ren Fail, 34(1), 111-118. doi: 10.3109/0886022X.2011.633446.
  • Carden D.L. & Granger D.N. (2000). Pathophysiology of ischaemia-reperfusion injury. J Pathol, 190(3), 255-66. doi: 10.1002/(SICI)10969896(200002)190:3<255::AID-PATH526>3.0.CO;2-6.
  • Ekim H., Erdoğan H.B., Kutay V., Başel H., Özen S., Hazar A, Akbayrak H. & Özbek H. (2005). Effect of ıschemia/reperfusion ınjury to the lungs caused by cross camping of the abdominal aorta. Van Tıp Dergisi, 12 (3),175-178.
  • Eltzschig H.K. & Collard C.D. (2004). Vascular ischaemia and reperfusion injury. Br Med Bull, 70, 71-86. doi:10.1093/bmb/ldh025
  • Erer D., Özer A., Demirtaş H., Gönül İ., Kara H., Arpacı H., Comu F.M., Oktar G.L., Arslan M. & Küçük A. (2016). Effects of alprostadil and iloprost on renal, lung, and skeletal muscle injury following hindlimb ischemia-reperfusion injury in rats. Drug Des Devel Ther, 19(10), 2651-2658. doi: 10.2147/DDDT.S110529.
  • Erre G.L. & Passiu G. (2009). Antioxidant effect of Iloprost: current knowledge and therapeutic implications for systemic sclerosis. Reumatismo, 61(2), 90-97. doi: 10.4081/reumatismo.2009.90.
  • Fileta J.B., Huang W., Kwon G.P., Filippopoulos T., Ben Y., Dobberfuhl A. & Grosskreutz C.L. (2008). Efficient estimation of retinal ganglion cell number: a stereological approach. Journal of neuroscience methods, 170(1), 1-8.
  • Holger K., Eltzschig H.K. & Eckle T. (2011). Ischemia and reperfusion—from mechanism to translation. NatMed, 17(11), 1391-1401. doi: 10.1038/nm.2507.
  • Huang C., Hong M.K. & Ding D.C. (2017). A review of ovary torsion. CiJiYiXueZaZhi, 29(3), 143-147. doi: 10.4103/tcmj.tcmj_55_17
  • Huang S.S., Wei F.C. & Hung L.M. (2006).Ischemic preconditioning attenuates postischemic leukocyte – endothelial cell interactions role of nitricoxide and protein kinase C. Circ J, 70:1070-1075.
  • Iriz E., Iriz A., Take G., Ozgul H., Oktar L., Demirtas H., Helvacıoğlu F. & Arslan M. (2015). Iloprostand vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits. Bratisl Lek Listy, 116(10), 627-631. doi: 10.4149/bll_2015_121.
  • Karaman S., Ozkan B., Yazir Y., Yardimoglu M., Gok M., Kara O., Vural C., Rencber S., Salih K. & Emek S.K. (2016). Comparison of hyperbaric oxygen versus iloprost treatment in an experimental rat central retinal artery occlusion model. Graefes Arch Clin Exp Ophthalmol, 254(11), 2209-2215. doi: 10.1007/s00417-016-3444-5.
  • Kart C., Aran T., Guven S., Karahan S.C. & Yulug E. (2011). Acute increase in plasma D-dimerlevel in ovarian torsion: an experimental study. Hum Reprod, 26(3), 564-568. doi: 10.1093/humrep/deq378.
  • McNamara D.B., Champion H.C. & Kadowitz PJ, (1998). Nonoperative management of lower extremity arterial disease. Part I. Pharmocologic management of peripheral vascular disease. Surg Clin North Am, 78, 447-456.
  • Nahar, N., Mohamed, S., Mustapha, N.M., Lau, S., Ishak, N.I.M. & Umran, N.S. (2021). Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemicrat model. Naunyn-Schmiedeberg'sArchives of Pharmacology, 394(3), 457-467.
  • Özat M., Gungor T., Barun S., Demirogullari B., Sokmensuer L.K., Gulbahar O., Gursoy D & Muftuoglu S. (2009). The effects of iloprost, a prostacyclin analo-gue, in experimental ischaemia/ reperfusion injury in rat ovaries. Experimental and Toxicologic Pathology, 61(5), 519-527.doi: 10.1016/j.etp.2009.06.007.
  • Özdemir G. & İnanc F. (2005). Zinc may protect remoteocular injury caused by intestinal ischemia reperfusion in rats. Tohoku J ExpMed, 206(3), 247-251. doi: 10.1620/tjem.206.247.
  • Rowlands T.E., Gough M.J. & Homer-Vanniasinkam S. (1999). Do prostaglandins have a salutary role in skeletal muscle ischaemia-reperfusion injury? Eur J Vasc Endovasc Surg, 18(5), 439-444. doi: 10.1053/ejvs.1999.0929.
  • Uludağ Ö. (2019). History and Importance of Ethical Rules in Animal Experiment Studies. Adıyaman Üniversitesi Sağlık Bilimleri Derg. 5(1), 1401–1413.
  • Thomson I.A., Egginton S., Slimms M.H. & Hudlicka O. (1996). Effect of muscle ischemia and iloprost during femoro distal reconstruction on capillary endothelial swelling. Int J Microcirc Clin Exp, 16, 284-290.
  • Wu M.Y. Yiang G.T., Liao W.T., Tsai A.P., Cheng Y.L., Cheng P.W., Li C.Y. & Li C.J. (2018). Current mechanistic concepts in ischemia and reperfusion injury. Cell Physiol Biochem, 46, 1650–1667. doi: 10.1159/000489241.
  • Yellon D.M. & Hausenloy D.J. (2007). Myocardial reperfusion injury. N Engl J Med, 357(11), 1121-1135. doi: 10 .1056/NEJMra071667
  • Yılmaz Y. & Tumkaya L. (2019). Effects of hyperbaric oxygen and iloprost on intestina lischemia-reperfusion induced acute lung injury. Ann Surg Treat Res, 96(1), 34-40. doi: 10.4174/astr.2019.96.1.34.
  • Yu H., Kalogeris T. & Korthuis R. (2019). Reactive species-induced microvascular dysfunction in ischemia/reperfusion. Free Radic Biol Med, 135,182-197. doi: 10.1016/j.freeradbiomed.2019.02.031.
  • Zhou M., Yu Y., Luo X., Wang J., Lan X., Liu P., Feng Y. & Jian W. (2021). Myocardial Ischemia-Reperfusion Injury: Therapeutics from a Mitochondria-Centric Perspective. Cardiology, 146(6), 781-792. doi: 10.1159/000518879.
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Primary Health Care
Journal Section Research Articles
Authors

Fadime Tosun 0000-0003-4660-5497

Ebru Annaç 0000-0001-9726-5846

Mevlüt Doğukan 0000-0002-4890-758X

Mehmet Duran 0000-0001-7568-3537

Öznur Uludag 0000-0002-6017-5836

Publication Date December 23, 2022
Submission Date September 20, 2022
Published in Issue Year 2022 Volume: 1 Issue: 3

Cite

APA Tosun, F., Annaç, E., Doğukan, M., Duran, M., et al. (2022). Over iskemi reperfüzyon hasarına karşı verilen iloprost infüzyonunun göz üzerine etkileri: ratlarda deneysel çalışma. Journal of Medical Topics and Updates, 1(3), 152-159.