Research Article
Metabolite Production and Antibacterial Activities of Callus Cultures from Rosa damascena Mill. Petals
Abstract
Rosa damascena Mill. is a very fragrant member of Rosaceae
family, exhibiting strong antioxidant and antimicrobial activity.
In this study, in vitro cell cultures from Rosa damascena Mill.
flower petals were established and the effects of precursor
L-phenylalanine, the elicitor methyl jasmonate and light/dark
treatments on the accumulation of characteristic rose volatile
compounds, tocopherols and β-carotene in addition to the
antibacterial activity of petal callus extracts were investigated
with Solid-Phase Microextraction Combined with Gas
Chromatography–Mass Spectrometry (GC–MS). The amount
of β-pinene increased by 236 times when 500 μM Phe was
added to the culture medium or under light treatment. Strong
antibacterial activity from petal callus extracts, up to 2.6-
fold compared to amikacin as well as original petal tissues,
was obtained under dark treatment for P. aeruginosa ATCC
27853 and S. aureus ATCC 25923. These results revealed that
petal callus extracts are sources of natural antioxidants and
antibacterial compounds.
References
- 1. Baydar H. Oil-bearing rose (Rosa damascena Mill.) cultivation and rose oil industry in Turkey. Euro Cosmetics 2006; 14: 13. 2. Kovatcheva-Apostolova EG, Georgiev MI, Ilieva MP, Skibsted LH, Rødtjer A, Andersen ML. Extracts of plant cell cultures of Lavandula vera and Rosa damascena as sources of phenolic antioxidants for use in foods. Eur Food Res Technol 2008; 227: 1243-9. 3. Sagdiç O, Baydar N, Baydar H. Note: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Sci Technol Int 2004; 10: 277-81. 4. Ulusoy S, Boşgelmez-Tınaz G, Seçilmiş-Canbay H. Tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute. Curr Microbiol 2009; 59: 554-8. Rosa damascena Mill. petal kallus kültürlerinin antibakteriyel etkileri ve metabolit üretimi ÖZ Rosa damascena Mill. Rosaceae familyasının güzel kokulu çiçekleri olan bir üyesi olup, güçlü antioksidan ve antimikrobiyal özelliğe sahiptir. Bu çalışmada Rosa damascena Mill. çiçek petallerinden in vitro hücre kültürü yapılmış, bu kültürlerden hazırlanan ekstrelerde L-fenilalanin, metil jasmonat, ışık/ karanlık uygulamasının gül uçucu bileşenleri, tokoferol, β-karoten üretimine etkisi ve antibakteriyel özellikleri incelenmiştir. Petal hücre ekstrelerinde, terpeneoidler (alfa/beta pinen) fenileten, kaproaldehit and benzaldehit bulunduğu SPME/GC-MS ile tespit edilmiştir. Işık veya 500 μM fenilalanin uygulaması ile beta pinen miktarının 236 kat arttığı belirlenmiştir. Karanlık uygulaması ile kültür ekstrelerinin antibakteriyel etkilerinin P. aeruginosa ATCC 27853 ve S. aureus ATCC 25923 için kanamisine göre 2.6 kat arttığı belirlenmiştir. Bu sonuçlar, petal kallus ekstrelerinin doğal antioksidan ve antibakteriyel moleküller içerdiğini göstermektedir. Anahtar kelimeler: Rosa damascena Mill., çiçek petalleri, kallus, sekonder metabolit, antibakteriyel aktivite 5. Da Porto C, Decorti D, Natolino A. Application of a supercritical CO2 extraction procedure to recover volatile compounds and polyphenols from Rosa damascena. Separ Sci Technol 2015; 50: 1175-80. 6. Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 2003; 74: 394-6. 7. Achuthan C, Babu B, Padikkala J. Antioxidant and hepatoprotective effects of Rosa damascena. Pharm Biol 2003; 41: 357-61. 8. Matkowski A. Plant in vitro culture for the production of antioxidants—a review. Biotechnol Adv 2008; 26: 548-60. 9. Roberts SC. Production and engineering of terpenoids in plant cell culture. Nature Chem Biol 2007; 3: 387-95. 10. Namdeo A. Plant cell elicitation for production of secondary metabolites: a review. Pharmacogn Rev 2007; 1: 69-79. 11. Skrzypczak-Pietraszek E, Słota J, Pietraszek J. The influence of L-phenylalanine, methyl jasmonate and sucrose concentration on the accumulation of phenolic acids in Exacum affine Balf. f. ex Regel shoot culture. Acta Biochim Pol 2014; 61: 47-53. 12. Arora R. Medicinal plant biotechnology: CABI; 2010. 13. Pavlov A, Popov S, Kovacheva E, Georgiev M, Ilieva M. Volatile and polar compounds in Rosa damascena Mill 1803 cell suspension. J Biotechnol 2005; 118: 89-97. 14. Banthorpe DV, Branch SA, Poots I, Fordham WD. Accumulation of 2-phenylethanol by callus derived from leafbud of Rosa damascena. Phytochem 1988; 27: 795-801. 15. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 1962; 15: 473-97. 16. Lampi A-M, Kataja L, Kamal-Eldin A, Vieno P. Antioxidant activities of α-and γ-tocopherols in the oxidation of rapeseed oil triacylglycerols. J Am Oil Chem Soc 1999; 76: 749-55. 17. Creelman RA, Mullet JE. Biosynthesis and action of jasmonates in plants. Annu Rev Plant Biol 1997; 48: 355-81. 18. Gala R, Mita G, Caretto S. Improving α-tocopherol production in plant cell cultures. J Plant Physiol 2005; 162: 782-4. 19. Yamamoto S, Hayashi S, Furusaki S, Shioya S. 5‐Aminolevulinic acid promotes callus growth and paclitaxel production in light‐grown Taxus cuspidata suspension cultures. Eng Life Sci 2015; 15: 116-21. 20. Zhong Jj, Seki T, Kinoshita Si, Yoshida T. Effect of light irradiation on anthocyanin production by suspended culture of Perilla frutescens. Biotechnol Bioeng 1991; 38: 653-8. 21. Guarnerio CF, Fraccaroli M, Gonzo I, Pressi G, Dal Toso R, Guzzo F, Levi M. Metabolomic analysis reveals that the accumulation of specific secondary metabolites in Echinacea angustifolia cells cultured in vitro can be controlled by light. Plant Cell Rep 2012; 31: 361-7. 22. Sooriamuthu S, Varghese RJ, Bayyapureddy A, John SST, Narayanan R. Light-induced production of antidepressant compounds in etiolated shoot cultures of Hypericum hookerianum Wight & Arn.(Hypericaceae). Plant Cell, Tissue Organ Cult (PCTOC) 2013; 115: 169-78. 23. Shinde AN, Malpathak N, Fulzele DP. Determination of isoflavone content and antioxidant activity in Psoralea corylifolia L. callus cultures. Food Chem 2010; 118: 128-32. 24. Dudareva N, Pichersky E, Gershenzon J. Biochemistry of plant volatiles. Plant Physiol 2004; 135: 1893-1902. 25. Köllner TG, Lenk C, Zhao N, Seidl-Adams I, Gershenzon J, Chen F, Degenhardt J. Herbivore-induced SABATH methyltransferases of maize that methylate anthranilic acid using S-adenosyl-L-methionine. Plant Physiol 2010; 153: 1795-1807. 26. Sreekumar S, Seeni S, Pushpangadan P. Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde. Plant Cell, Tissue Organ Cult 2000; 62: 211-8.
Year 2017,
Volume: 21 Issue: 3, 590 - 597, 20.06.2017
Abstract
References
- 1. Baydar H. Oil-bearing rose (Rosa damascena Mill.) cultivation and rose oil industry in Turkey. Euro Cosmetics 2006; 14: 13. 2. Kovatcheva-Apostolova EG, Georgiev MI, Ilieva MP, Skibsted LH, Rødtjer A, Andersen ML. Extracts of plant cell cultures of Lavandula vera and Rosa damascena as sources of phenolic antioxidants for use in foods. Eur Food Res Technol 2008; 227: 1243-9. 3. Sagdiç O, Baydar N, Baydar H. Note: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Sci Technol Int 2004; 10: 277-81. 4. Ulusoy S, Boşgelmez-Tınaz G, Seçilmiş-Canbay H. Tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute. Curr Microbiol 2009; 59: 554-8. Rosa damascena Mill. petal kallus kültürlerinin antibakteriyel etkileri ve metabolit üretimi ÖZ Rosa damascena Mill. Rosaceae familyasının güzel kokulu çiçekleri olan bir üyesi olup, güçlü antioksidan ve antimikrobiyal özelliğe sahiptir. Bu çalışmada Rosa damascena Mill. çiçek petallerinden in vitro hücre kültürü yapılmış, bu kültürlerden hazırlanan ekstrelerde L-fenilalanin, metil jasmonat, ışık/ karanlık uygulamasının gül uçucu bileşenleri, tokoferol, β-karoten üretimine etkisi ve antibakteriyel özellikleri incelenmiştir. Petal hücre ekstrelerinde, terpeneoidler (alfa/beta pinen) fenileten, kaproaldehit and benzaldehit bulunduğu SPME/GC-MS ile tespit edilmiştir. Işık veya 500 μM fenilalanin uygulaması ile beta pinen miktarının 236 kat arttığı belirlenmiştir. Karanlık uygulaması ile kültür ekstrelerinin antibakteriyel etkilerinin P. aeruginosa ATCC 27853 ve S. aureus ATCC 25923 için kanamisine göre 2.6 kat arttığı belirlenmiştir. Bu sonuçlar, petal kallus ekstrelerinin doğal antioksidan ve antibakteriyel moleküller içerdiğini göstermektedir. Anahtar kelimeler: Rosa damascena Mill., çiçek petalleri, kallus, sekonder metabolit, antibakteriyel aktivite 5. Da Porto C, Decorti D, Natolino A. Application of a supercritical CO2 extraction procedure to recover volatile compounds and polyphenols from Rosa damascena. Separ Sci Technol 2015; 50: 1175-80. 6. Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 2003; 74: 394-6. 7. Achuthan C, Babu B, Padikkala J. Antioxidant and hepatoprotective effects of Rosa damascena. Pharm Biol 2003; 41: 357-61. 8. Matkowski A. Plant in vitro culture for the production of antioxidants—a review. Biotechnol Adv 2008; 26: 548-60. 9. Roberts SC. Production and engineering of terpenoids in plant cell culture. Nature Chem Biol 2007; 3: 387-95. 10. Namdeo A. Plant cell elicitation for production of secondary metabolites: a review. Pharmacogn Rev 2007; 1: 69-79. 11. Skrzypczak-Pietraszek E, Słota J, Pietraszek J. The influence of L-phenylalanine, methyl jasmonate and sucrose concentration on the accumulation of phenolic acids in Exacum affine Balf. f. ex Regel shoot culture. Acta Biochim Pol 2014; 61: 47-53. 12. Arora R. Medicinal plant biotechnology: CABI; 2010. 13. Pavlov A, Popov S, Kovacheva E, Georgiev M, Ilieva M. Volatile and polar compounds in Rosa damascena Mill 1803 cell suspension. J Biotechnol 2005; 118: 89-97. 14. Banthorpe DV, Branch SA, Poots I, Fordham WD. Accumulation of 2-phenylethanol by callus derived from leafbud of Rosa damascena. Phytochem 1988; 27: 795-801. 15. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 1962; 15: 473-97. 16. Lampi A-M, Kataja L, Kamal-Eldin A, Vieno P. Antioxidant activities of α-and γ-tocopherols in the oxidation of rapeseed oil triacylglycerols. J Am Oil Chem Soc 1999; 76: 749-55. 17. Creelman RA, Mullet JE. Biosynthesis and action of jasmonates in plants. Annu Rev Plant Biol 1997; 48: 355-81. 18. Gala R, Mita G, Caretto S. Improving α-tocopherol production in plant cell cultures. J Plant Physiol 2005; 162: 782-4. 19. Yamamoto S, Hayashi S, Furusaki S, Shioya S. 5‐Aminolevulinic acid promotes callus growth and paclitaxel production in light‐grown Taxus cuspidata suspension cultures. Eng Life Sci 2015; 15: 116-21. 20. Zhong Jj, Seki T, Kinoshita Si, Yoshida T. Effect of light irradiation on anthocyanin production by suspended culture of Perilla frutescens. Biotechnol Bioeng 1991; 38: 653-8. 21. Guarnerio CF, Fraccaroli M, Gonzo I, Pressi G, Dal Toso R, Guzzo F, Levi M. Metabolomic analysis reveals that the accumulation of specific secondary metabolites in Echinacea angustifolia cells cultured in vitro can be controlled by light. Plant Cell Rep 2012; 31: 361-7. 22. Sooriamuthu S, Varghese RJ, Bayyapureddy A, John SST, Narayanan R. Light-induced production of antidepressant compounds in etiolated shoot cultures of Hypericum hookerianum Wight & Arn.(Hypericaceae). Plant Cell, Tissue Organ Cult (PCTOC) 2013; 115: 169-78. 23. Shinde AN, Malpathak N, Fulzele DP. Determination of isoflavone content and antioxidant activity in Psoralea corylifolia L. callus cultures. Food Chem 2010; 118: 128-32. 24. Dudareva N, Pichersky E, Gershenzon J. Biochemistry of plant volatiles. Plant Physiol 2004; 135: 1893-1902. 25. Köllner TG, Lenk C, Zhao N, Seidl-Adams I, Gershenzon J, Chen F, Degenhardt J. Herbivore-induced SABATH methyltransferases of maize that methylate anthranilic acid using S-adenosyl-L-methionine. Plant Physiol 2010; 153: 1795-1807. 26. Sreekumar S, Seeni S, Pushpangadan P. Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde. Plant Cell, Tissue Organ Cult 2000; 62: 211-8.
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Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 20, 2017 |
| Published in Issue | Year 2017 Volume: 21 Issue: 3 |