Araştırma Makalesi
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Van Balığında (Alburnus tarichi Guldenstadt, 1814) Üreme Göçü Sırasında Oksidatif Stresin Araştırılması

Yıl 2019, Cilt: 8 Sayı: 1, 90 - 97, 12.03.2019
https://doi.org/10.17798/bitlisfen.512275

Öz

Van Balığı, Türkiye’nin en büyük gölüne
endemik anadrom bir türdür. Balıklar, her sene üremesini gerçekleştirmek için
alkalin Van Gölünden göle dökülen tatlı sulara göç ederler. Balık bu göç
sırasında açlık, tuz ve pH adaptasyonu gibi farklı stres faktörlerine maruz
kalır. Bu çalışmada, Van Balığının üreme göçü öncesi ve sonrasında plazma, solungaç,
karaciğer, beyin ve kas gibi dokularindaki total oksidan ve antioksidan, lipid
peroksidasyon ve DNA hasarları karşılaştırıldı. Total oksidan durum (TOS) üreme
öncesi gölden yakalanan balıkların karaciğer ve böbrek dokularında, total
antioksidan durum (TAS) ise akarsudan yakalanan balıklarda karaciğerde, gölden
yakalanan balıkların ise beyin ve böbrek dokularında yüksek olduğu gözlendi
(P<0.05). Malondialdehit (MDA) seviyelerinin akarsudan örneklenen balıkların
karaciğerinde gölden örneklenen balıklarda ise böbreklerinde daha fazla olduğu
belirlendi (P<0.05). DNA hasarınin ise sadece tatlı sudan örneklenen
balıkların karaciğerinde arttığı belirlendi. Sonuç olarak, Van Balığında üreme
göçü sırasında karşılaşılan stres etkilerine karşı organların gösterdiği
tepkiler belirlendi. 

Kaynakça

  • 1. Lushchak V.I. 2011. Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology, 101(1): 13-30.
  • 2. Birnie‐Gauvin K., Costantini D., Cooke S.J., Willmore W.G. 2017. A comparative and evolutionary approach to oxidative stress in fish: a review. Fish and Fisheries, 18(5): 928-942.
  • 3. Kasai H. 1997. Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutation Research/Reviews in Mutation Research, 387(3): 147-163.
  • 4. Almroth B.C., Asker N., Wassmur B., Rosengren M., Jutfelt F., Gräns A., Sundell K., Axelsson M., Sturve J. 2015. Warmer water temperature results in oxidative damage in an Antarctic fish, the bald notothen. Journal of Experimental Marine Biology and Ecology, 468: 130-137.
  • 5. Wilson S.M., Taylor J.J., Mackie T.A., Patterson D.A., Cooke S.J., Willmore W.G. 2014. Oxidative stress in Pacific salmon (Oncorhynchus spp.) during spawning migration. Physiological and Biochemical Zoology, 87(2): 346-352.
  • 6. Liu Y., Wang W.N., Wang A.L., Wang J.M., Sun R.Y. 2007. Effects of dietary vitamin E supplementation on antioxidant enzyme activities in Litopenaeus vannamei (Boone, 1931) exposed to acute salinity changes. Aquaculture, 265(1-4): 351-358.
  • 7. Bayir A., Sirkecioglu A.N., Bayir M., Haliloglu H.I., Kocaman E.M., Aras N.M. 2011. Metabolic responses to prolonged starvation, food restriction, and refeeding in the brown trout, Salmo trutta: oxidative stress and antioxidant defenses. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 159(4): 191-196.
  • 8. Morales A.E., Pérez-Jiménez A., Hidalgo M.C., Abellán E., Cardenete G. 2004. Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 139(1): 153-161.
  • 9. Rueda-Jasso R., Conceiçao L.E., Dias J., De Coen W., Gomes E., Rees J.F., Sorgeloos P. 2004. Effect of dietary non-protein energy levels on condition and oxidative status of Senegalese sole (Solea senegalensis) juveniles. Aquaculture, 231(1-4): 417-433.
  • 10. Danulat E, Selçuk B. 1992. Life history and enviromental conditions of the anadromous Chalcalburnus tarichi (Cyprinidae) in the highly alkaline Lake Van, Eastern Anatolia, Turkey. Arch Hidrobiol.126(1): 105–125.
  • 11. Kelly K.A., Havrilla C.M., Brady T.C., Abramo K.H., Levin E.D. 1998. Oxidative stress in toxicology: established mammalian and emerging piscine model systems. Environmental health perspectives, 106(7): 375-384.
  • 12. Slaninova A., Smutna M., Modra H., Svobodova Z. 2009. REVIEWS Oxidative stress in fish induced by pesticides. Neuroendocrinology Letters, 30(1), 2.
  • 13. Jain S.K., McVie R., Duett J., Herbst J.J. 1989. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes, 38(12): 1539-1543.
  • 14. Ünal G., Çetinkaya O., Elp M. 1999. Histological investigation of gonad development of Chalcalburnus tarichi (P., 1811). Turkish Journal of Zoology, 23(EK1): 329-338. 15. Marshall W.S., Grosell M. 2006. Ion transport, osmoregulation, and acid-base balance. The physiology of fishes, 3: 177-230.
  • 16. Pascual P., Pedrajas J.R., Toribio F., López-Barea J., Peinado J. 2003. Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chemico-biological interactions, 145(2): 191-199.
  • 17. Nam Y.K., Cho Y.S., Choi B.N., Kim K.H., Kim S.K., Kim D.S. 2005. Alteration of antioxidant enzymes at the mRNA level during short‐term starvation of rockbream Oplegnathus fasciatus. Fisheries Science, 71(6): 1385-1387.
  • 18. Welker T.L., Congleton J.L. 2005. Oxidative stress in migrating spring Chinook salmon smolts of hatchery origin: changes in vitamin E and lipid peroxidation. Transactions of the American Fisheries Society, 134(6): 1499-1508.
  • 19. Hidalgo M.C., Exposito A., Palma J.M., de la Higuera M. 2002. Oxidative stress generated by dietary Zn-deficiency: studies in rainbow trout (Oncorhynchus mykiss). The international journal of biochemistry & cell biology, 34(2): 183-193.
  • 20. Varju M., Müller T., Bokor Z., Żarski D., Mézes M., Balogh K. 2018. The effects of excessive starvation on antioxidant defence and lipid peroxidation in intensively reared, commercial-size pikeperch (Sander lucioperca L.). The Egyptian Journal of Aquatic Research, 44(4): 349-352.
  • 21. Martinez-Alvarez R.M., Hidalgo M.C., Domezain A., Morales A.E., García-Gallego M., Sanz A. 2002. Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of experimental biology, 205(23): 3699-3706.
  • 22. Martínez-Álvarez R.M., Morales A.E., Sanz A. 2005. Antioxidant defenses in fish: biotic and abiotic factors. Reviews in Fish Biology and fisheries, 15(1-2): 75-88.
  • 23. Barzilai A., Yamamoto, K.I. 2004. DNA damage responses to oxidative stress. DNA Repair, 3(8-9): 1109-1115.
  • 24. Oğuz A.R. 2015. Histological changes in the gill epithelium of endemic Lake Van Fish (Chalcalburnus tarichi) during migration from alkaline water to freshwater, North-Western Journal of Zoology 11(1):51-57.
  • 25. Oğuz A.R. 2013. Environmental regulation of mitochondria-rich cells in Chalcalburnus tarichi (Pallas, 1811) during reproductive migration. The Journal of membrane biology, 246(3): 183-188.
  • 26. Miller K.M., Schulze A.D., Ginther N., Li S., Patterson D.A., Farrell A.P., Hinch S.G. 2009. Salmon spawning migration: metabolic shifts and environmental triggers. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 4(2): 75-89.

Investigation of Oxidative Stress in Van Fish (Alburnus tarichi Guldenstadt, 1814) During Reproductive Migration

Yıl 2019, Cilt: 8 Sayı: 1, 90 - 97, 12.03.2019
https://doi.org/10.17798/bitlisfen.512275

Öz












Van Fish is an anadromous species of endemic to
Turkey's largest lake. The fish migrate to the freshwater pouring from the
alkaline Lake Van to the lake for the reproduction every year. Fish are exposed
to different stress factors such as fasting, salt and pH adaptation during
migration. In this study, total oxidant and antioxidant, lipid peroxidation and
DNA damages in tissues such as gill, liver, plasma, brain and muscle were
compared before and after reproductive migration. Total oxidant status (TOS) of
the fish caught in the lake before reproduction, liver and kidney tissues,
total antioxidant status (TAS), fish liver caught from freshwater, fish from
the lake were observed to be high in the brain and kidney tissues (P <0.05).
Malondialdehyde (MDA) levels were found to be higher in the liver and kidney of
the fish sampled from the freshwater and lake, respectively. (P <0.05). DNA
damage was found only in the liver of fish sampled from freshwater. As a result,
the reactions of the organs against stress effects encountered during
reproduction migration were determined.

Kaynakça

  • 1. Lushchak V.I. 2011. Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology, 101(1): 13-30.
  • 2. Birnie‐Gauvin K., Costantini D., Cooke S.J., Willmore W.G. 2017. A comparative and evolutionary approach to oxidative stress in fish: a review. Fish and Fisheries, 18(5): 928-942.
  • 3. Kasai H. 1997. Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutation Research/Reviews in Mutation Research, 387(3): 147-163.
  • 4. Almroth B.C., Asker N., Wassmur B., Rosengren M., Jutfelt F., Gräns A., Sundell K., Axelsson M., Sturve J. 2015. Warmer water temperature results in oxidative damage in an Antarctic fish, the bald notothen. Journal of Experimental Marine Biology and Ecology, 468: 130-137.
  • 5. Wilson S.M., Taylor J.J., Mackie T.A., Patterson D.A., Cooke S.J., Willmore W.G. 2014. Oxidative stress in Pacific salmon (Oncorhynchus spp.) during spawning migration. Physiological and Biochemical Zoology, 87(2): 346-352.
  • 6. Liu Y., Wang W.N., Wang A.L., Wang J.M., Sun R.Y. 2007. Effects of dietary vitamin E supplementation on antioxidant enzyme activities in Litopenaeus vannamei (Boone, 1931) exposed to acute salinity changes. Aquaculture, 265(1-4): 351-358.
  • 7. Bayir A., Sirkecioglu A.N., Bayir M., Haliloglu H.I., Kocaman E.M., Aras N.M. 2011. Metabolic responses to prolonged starvation, food restriction, and refeeding in the brown trout, Salmo trutta: oxidative stress and antioxidant defenses. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 159(4): 191-196.
  • 8. Morales A.E., Pérez-Jiménez A., Hidalgo M.C., Abellán E., Cardenete G. 2004. Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 139(1): 153-161.
  • 9. Rueda-Jasso R., Conceiçao L.E., Dias J., De Coen W., Gomes E., Rees J.F., Sorgeloos P. 2004. Effect of dietary non-protein energy levels on condition and oxidative status of Senegalese sole (Solea senegalensis) juveniles. Aquaculture, 231(1-4): 417-433.
  • 10. Danulat E, Selçuk B. 1992. Life history and enviromental conditions of the anadromous Chalcalburnus tarichi (Cyprinidae) in the highly alkaline Lake Van, Eastern Anatolia, Turkey. Arch Hidrobiol.126(1): 105–125.
  • 11. Kelly K.A., Havrilla C.M., Brady T.C., Abramo K.H., Levin E.D. 1998. Oxidative stress in toxicology: established mammalian and emerging piscine model systems. Environmental health perspectives, 106(7): 375-384.
  • 12. Slaninova A., Smutna M., Modra H., Svobodova Z. 2009. REVIEWS Oxidative stress in fish induced by pesticides. Neuroendocrinology Letters, 30(1), 2.
  • 13. Jain S.K., McVie R., Duett J., Herbst J.J. 1989. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes, 38(12): 1539-1543.
  • 14. Ünal G., Çetinkaya O., Elp M. 1999. Histological investigation of gonad development of Chalcalburnus tarichi (P., 1811). Turkish Journal of Zoology, 23(EK1): 329-338. 15. Marshall W.S., Grosell M. 2006. Ion transport, osmoregulation, and acid-base balance. The physiology of fishes, 3: 177-230.
  • 16. Pascual P., Pedrajas J.R., Toribio F., López-Barea J., Peinado J. 2003. Effect of food deprivation on oxidative stress biomarkers in fish (Sparus aurata). Chemico-biological interactions, 145(2): 191-199.
  • 17. Nam Y.K., Cho Y.S., Choi B.N., Kim K.H., Kim S.K., Kim D.S. 2005. Alteration of antioxidant enzymes at the mRNA level during short‐term starvation of rockbream Oplegnathus fasciatus. Fisheries Science, 71(6): 1385-1387.
  • 18. Welker T.L., Congleton J.L. 2005. Oxidative stress in migrating spring Chinook salmon smolts of hatchery origin: changes in vitamin E and lipid peroxidation. Transactions of the American Fisheries Society, 134(6): 1499-1508.
  • 19. Hidalgo M.C., Exposito A., Palma J.M., de la Higuera M. 2002. Oxidative stress generated by dietary Zn-deficiency: studies in rainbow trout (Oncorhynchus mykiss). The international journal of biochemistry & cell biology, 34(2): 183-193.
  • 20. Varju M., Müller T., Bokor Z., Żarski D., Mézes M., Balogh K. 2018. The effects of excessive starvation on antioxidant defence and lipid peroxidation in intensively reared, commercial-size pikeperch (Sander lucioperca L.). The Egyptian Journal of Aquatic Research, 44(4): 349-352.
  • 21. Martinez-Alvarez R.M., Hidalgo M.C., Domezain A., Morales A.E., García-Gallego M., Sanz A. 2002. Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of experimental biology, 205(23): 3699-3706.
  • 22. Martínez-Álvarez R.M., Morales A.E., Sanz A. 2005. Antioxidant defenses in fish: biotic and abiotic factors. Reviews in Fish Biology and fisheries, 15(1-2): 75-88.
  • 23. Barzilai A., Yamamoto, K.I. 2004. DNA damage responses to oxidative stress. DNA Repair, 3(8-9): 1109-1115.
  • 24. Oğuz A.R. 2015. Histological changes in the gill epithelium of endemic Lake Van Fish (Chalcalburnus tarichi) during migration from alkaline water to freshwater, North-Western Journal of Zoology 11(1):51-57.
  • 25. Oğuz A.R. 2013. Environmental regulation of mitochondria-rich cells in Chalcalburnus tarichi (Pallas, 1811) during reproductive migration. The Journal of membrane biology, 246(3): 183-188.
  • 26. Miller K.M., Schulze A.D., Ginther N., Li S., Patterson D.A., Farrell A.P., Hinch S.G. 2009. Salmon spawning migration: metabolic shifts and environmental triggers. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 4(2): 75-89.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Ahmet Regaib Oğuz 0000-0001-6431-0508

Necati Özok

Aso Omar Bu kişi benim

Zehra Alkan

Ayşe Nur Kıraççakalı Bu kişi benim

Yayımlanma Tarihi 12 Mart 2019
Gönderilme Tarihi 13 Ocak 2019
Kabul Tarihi 21 Ocak 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 8 Sayı: 1

Kaynak Göster

IEEE A. R. Oğuz, N. Özok, A. Omar, Z. Alkan, ve A. N. Kıraççakalı, “Investigation of Oxidative Stress in Van Fish (Alburnus tarichi Guldenstadt, 1814) During Reproductive Migration”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, c. 8, sy. 1, ss. 90–97, 2019, doi: 10.17798/bitlisfen.512275.



Bitlis Eren Üniversitesi
Fen Bilimleri Dergisi Editörlüğü

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