Öz
Nowadays, due to the stressful, tiring and busy lives of humans, the immune system becomes weak and can get sick easily. Therefore, scientists have been doing researches about new, natural and healthy products that can strengthen their immune system and can provide to adapt life standards. One of these products is the beta glucan. It is a polysaccharide molecule that consists of D-glucose monomers bonding with beta glycosidic bonds. Beta (β) glucans have being produced from different sources (microorganisms, cereals and mushrooms) so they have different branched structures such as (1→3) (1→6), (1→3) (1→4), (1→3) (1→2). Thus, different branched beta glucans show different physicochemical properties and biological activities that designate their usage purposes. Especially yeast beta glucan has lots of biological activities. On the other hand, it is fact that the extraction method affects the molecular weight, yield, purity and other properties of beta glucans. The main purpose of this study is to compare the performances of ultrasonically assisted alkali-acidic and autolysis extraction methods to produce a high yield of beta glucan. Also, it was aimed to determine the effect of extraction method on the molecular weight of yeast beta glucan. As a result of this work, it was found that the yeast beta glucan yield for ultrasonic supported autolysis extraction (12 %) is higher than that of the ultrasonic supported alkali-acidic extraction (8 %). On the other hand, having the lower molecular weight of yeast beta glucan (87 kDa) was produced by using an ultrasonic supported autolysis extraction method.
Anahtar Kelimeler
Beta glucan Saccharomyces cerevisiae yeast alkali-acidic extraction autolysis molecular weight
Destekleyen Kurum
Ankara University Research Fund
Proje Numarası
17H0443006
Kaynakça
- Adachi M, Kowhakul W, Masamoto H, Shigematsu, M. 2013. Bioactivities of β-glucan and tannin extracted with superheated water by using a macchinetta extractor. 4th international conference on biology, environment and chemistry. 58. 71-76.
- Du B, Zhu F, Xu B. 2014. β-Glucan extraction from bran of hull-less barley by accelerated solvent extraction combined with response surface methodology. Journal of Cereal Science 59:95-100.
- Karimi R, Azizi MH, Xu Q. 2019. Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan. International Journal of Biological Macromolecules 126:298-309.
- Khan AA, Gani A, Masoodi FA, Mushtaq U, Naik AZ. 2017. Structural, rheological, antioxidant, and functional properties of β-D glucan extracted from edible mushrooms Agaricus bisporus, Pleurotus ostreatus and Coprinus attrimentarius. Bioactive Carbohydrates and Dietary Fibre 11:67-74.
- Khan AA, Gani A, Masoodi FA, Amin F, Wani IA, Khanday FA, Gani A. 2016. Structural, thermal, functional, antioxidant & antimicrobial properties of β-D glucan extracted from baker’s yeast (Saccharomyces cerevisiae) – Effect of ϫ- irradition. Carbohydrate polymers 140:442-450.
- Kim HJ, White PJ. 2013. Impact of the Molecular Weight, Viscosity, and Solubility of β – Glucan on in Vitro Oat Starch Digestibility. Journal of Agricultural and Food Chemistry 61:3270-3277.
- Klis FM, Mol P, Hellingwerf K, Brul S. 2002. Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiology Reviews 26:239-256.
- Kofuji K, Aoki A, Tsubaki K, Konishi M, Isobe T, Murata Y. 2012. Antioxidant Activity of β-Glucan. International Scholarly Research Network Pharmaceutics Article ID 125864, 1-5.
- Maheshwari G, Sowrirajan S, Joseph B. 2017. Extraction and Isolation of β-Glucan from Grain Sources-A review. Journal of Food Science 7:1535-1545.
- Mantovani MS Bellini MF, Angeli JPF, Oliveira RJ Silva, AF, Ribeiro LR. 2008. β-Glucans in promoting health: Prevention againts mutation and cancer, Reviews in Mutation Research 658:154-161.
- Özcan Ö, Ertan F. 2018. Beta-glucan Content, Antioxidant and Antimicrobial Activities of Some Edible Mushroom Species. Food Science and Technology 6(2):47-55.
- Sabuncu N. 2016. Investigation of growth conditions and pH control in a bioreactor to increase β-glucan content of S. cerevisiae. Master Thesis, Ankara University Graduate School of Natural and Applied Sciences Chemical Engineering Department, 140, Ankara.
- Sapirstein, HD. 2016. Bioactive Compounds in Wheat Bran. Encyclopedia of Food Grains (Second Edition) 2:268-276.
- Synytsya A, Novak M. 2014. Structural analysis of glucans. Ann Transl Med 2(2):17. doi:10.3978/j.issn.2305-5839.2014.02.07
- Stone, Bruce. 2009. Chemistry of β-Glucans. Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides. 5-46. 10.1016/B978-0-12-373971-1.00002-9.
- Valasques-Junior GL, de Lima FO, Boffo EF, Santoz JTG, da Silva BC, de Assis SA. 2014. Extraction optimization and antinociceptive activity of (1→3)-β-D-glucan from Rhodotorula mucilaginosa. Carbohydrate Polymers 105:293-299.
- Volpato H, Scariot DB, Soares EFP, Jacomini AP, Rosa FA, Sarragiotto MH, Ueda-Nakamura T, Rubira AF, Pereira GM, Manadas R, Leitao AJ, Borges O, Nakamura CV, do Ceu Sousa M. 2018. In vitro anti-Leishmania activity of T6 synthetic compound encapsulated in yeast-derived β-(1,3)-D-glucan particles. International Journal of Biological Macromolecules 119:1264-1275.
- Yılmaz C. 2010. Extraction of Glucan from Saccharomyces cerevisiae. Master Thesis, Kocaeli University Graduate School of Natural and Applied Sciences, Biology Department, 117, Kocaeli.
- Zhu H, Du B, Xu B. 2016. A critical review on production and industrial applications of beta-glucans. Food Hydrocolloids 52:275-288.
Öz
Proje Numarası
17H0443006
Kaynakça
- Adachi M, Kowhakul W, Masamoto H, Shigematsu, M. 2013. Bioactivities of β-glucan and tannin extracted with superheated water by using a macchinetta extractor. 4th international conference on biology, environment and chemistry. 58. 71-76.
- Du B, Zhu F, Xu B. 2014. β-Glucan extraction from bran of hull-less barley by accelerated solvent extraction combined with response surface methodology. Journal of Cereal Science 59:95-100.
- Karimi R, Azizi MH, Xu Q. 2019. Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan. International Journal of Biological Macromolecules 126:298-309.
- Khan AA, Gani A, Masoodi FA, Mushtaq U, Naik AZ. 2017. Structural, rheological, antioxidant, and functional properties of β-D glucan extracted from edible mushrooms Agaricus bisporus, Pleurotus ostreatus and Coprinus attrimentarius. Bioactive Carbohydrates and Dietary Fibre 11:67-74.
- Khan AA, Gani A, Masoodi FA, Amin F, Wani IA, Khanday FA, Gani A. 2016. Structural, thermal, functional, antioxidant & antimicrobial properties of β-D glucan extracted from baker’s yeast (Saccharomyces cerevisiae) – Effect of ϫ- irradition. Carbohydrate polymers 140:442-450.
- Kim HJ, White PJ. 2013. Impact of the Molecular Weight, Viscosity, and Solubility of β – Glucan on in Vitro Oat Starch Digestibility. Journal of Agricultural and Food Chemistry 61:3270-3277.
- Klis FM, Mol P, Hellingwerf K, Brul S. 2002. Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiology Reviews 26:239-256.
- Kofuji K, Aoki A, Tsubaki K, Konishi M, Isobe T, Murata Y. 2012. Antioxidant Activity of β-Glucan. International Scholarly Research Network Pharmaceutics Article ID 125864, 1-5.
- Maheshwari G, Sowrirajan S, Joseph B. 2017. Extraction and Isolation of β-Glucan from Grain Sources-A review. Journal of Food Science 7:1535-1545.
- Mantovani MS Bellini MF, Angeli JPF, Oliveira RJ Silva, AF, Ribeiro LR. 2008. β-Glucans in promoting health: Prevention againts mutation and cancer, Reviews in Mutation Research 658:154-161.
- Özcan Ö, Ertan F. 2018. Beta-glucan Content, Antioxidant and Antimicrobial Activities of Some Edible Mushroom Species. Food Science and Technology 6(2):47-55.
- Sabuncu N. 2016. Investigation of growth conditions and pH control in a bioreactor to increase β-glucan content of S. cerevisiae. Master Thesis, Ankara University Graduate School of Natural and Applied Sciences Chemical Engineering Department, 140, Ankara.
- Sapirstein, HD. 2016. Bioactive Compounds in Wheat Bran. Encyclopedia of Food Grains (Second Edition) 2:268-276.
- Synytsya A, Novak M. 2014. Structural analysis of glucans. Ann Transl Med 2(2):17. doi:10.3978/j.issn.2305-5839.2014.02.07
- Stone, Bruce. 2009. Chemistry of β-Glucans. Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides. 5-46. 10.1016/B978-0-12-373971-1.00002-9.
- Valasques-Junior GL, de Lima FO, Boffo EF, Santoz JTG, da Silva BC, de Assis SA. 2014. Extraction optimization and antinociceptive activity of (1→3)-β-D-glucan from Rhodotorula mucilaginosa. Carbohydrate Polymers 105:293-299.
- Volpato H, Scariot DB, Soares EFP, Jacomini AP, Rosa FA, Sarragiotto MH, Ueda-Nakamura T, Rubira AF, Pereira GM, Manadas R, Leitao AJ, Borges O, Nakamura CV, do Ceu Sousa M. 2018. In vitro anti-Leishmania activity of T6 synthetic compound encapsulated in yeast-derived β-(1,3)-D-glucan particles. International Journal of Biological Macromolecules 119:1264-1275.
- Yılmaz C. 2010. Extraction of Glucan from Saccharomyces cerevisiae. Master Thesis, Kocaeli University Graduate School of Natural and Applied Sciences, Biology Department, 117, Kocaeli.
- Zhu H, Du B, Xu B. 2016. A critical review on production and industrial applications of beta-glucans. Food Hydrocolloids 52:275-288.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Kimya Mühendisliği |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | 17H0443006 |
| Yayımlanma Tarihi | 26 Aralık 2021 |
| Kabul Tarihi | 9 Aralık 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 4 Sayı: 2 |
Kaynak Göster
