Araştırma Makalesi
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Yıl 2024, Cilt: 38 Sayı: 1, 140 - 146, 30.04.2024

Serol Korkmaz Hanife Banu Aydın İrem Korkmaz

Öz

Kaynakça

  • Alp E, Aksu MI (2010). Effects of water extract of Urtica dioica L. and modified atmosphere packaging on the shelf life of ground beef. Meat Science, 86(2): 468–473. Doi:10.1016/j.meatsci.2010.05.036
  • Aydin HB, Korkmaz S, Omurtag Korkmaz BI (2023). Antibacterial and antiviral activities of stinging nettle (Urtica dioica L.) leaf extract on norovirus and campylobacter jejuni as foodborne pathogens. Journal of Microbiology, Biotechnology and Food Sciences, 13(1):9768. Doi:10.55251/jmbfs.9768
  • Carvalho AR, Costa G, Figueirinha A, Liberal J, Prior JAV, Lopes MC, Cruz MT, Batista MT (2017). Urtica spp.: Phenolic composition, safety, antioxidant and anti-inflammatory activities. Food Research International, 99: 485–494. Doi:10.1016/j.foodres.2017.06.008
  • Dhouibi R, Affes H, Salem M Ben, Hammami S, Sahnoun Z, Zeghal KM, Ksouda K (2020). Screening of pharmacological uses of Urtica dioica and others benefits. Progress in Biophysics and Molecular Biology, 150: 67–77. Doi:10.1016/j.pbiomolbio.2019.05.008
  • Dini I (2019). An overview of functional beverages. Woodhead Publishing, MA, USA. p.1–40. Doi:10.1016/B978-0-12-816397-9.00001-7
  • Durak I, Biri H, Devrim E, Sözen S, Avcı A (2004). Aqueous extract of urtica dioica makes significant inhibition on adenosine deaminase activity in prostate tissue from patients with prostate cancer. Cancer Biology & Therapy, 3(9): 855–857. Doi:10.4161/cbt.3.9.1038
  • El-Saber Batiha G, Hussein DE, Algammal AM, George TT, Jeandet P, Al-Snafi AE, Tiwari A, Pagnossa JP, Lima CM, Thorat ND, Zahoor M, El-Esawi M, Dey A, Alghamdi S, Hetta HF, Cruz-Martins N (2021). Application of natural antimicrobials in food preservation: Recent views. Food Control, 126. Doi:10.1016/j.foodcont.2021.108066
  • Esposito S, Bianco A, Russo R, Maro A Di, Isernia C, Pedone PV (2019). Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer Treatment. Molecules, 24(15). Doi:10.3390/molecules24152753
  • European Commission (2023) Novel Food Catalogue. https://webgate.ec.europa.eu/fip/novel_food_catalogue/ (accessed date: 28.09.2023).
  • Fattahi S, Ardekani AM, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, Akhavan-Niaki H (2013). Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line. Asian Pacific Journal of Cancer Prevention, 14(9): 5317–5323
  • Fattahi S, Ghadami E, Asouri M, Ardekanid AM, Akhavan-Niaki H (2018). Urtica dioica inhibits cell growth and induces apoptosis by targeting Ornithine decarboxylase and Adenosine deaminase as key regulatory enzymes in adenosine and polyamines homeostasis in human breast cancer cell lines. Cellular and Molecular Biology, 64(3): 97–102
  • Flores-Ocelotl MR, Rosas-Murrieta NH, Moreno DA, Vallejo-Ruiz V, Reyes-Leyva J, Domínguez F, Santos-López G (2018). Taraxacum officinale and Urtica dioica extracts inhibit dengue virus serotype 2 replication in vitro. BMC complementary and alternative medicine, 18(1): 95. Doi:10.1186/s12906-018-2163-3
  • Garcìa LM, Ceccanti C, Negro C, Bellis L De, Incrocci L, Pardossi A, Guidi L (2021). Effect of drying methods on phenolic compounds and antioxidant activity of Urtica dioica L. leaves. Horticulturae, 7(1): 1–12. Doi:10.3390/horticulturae7010010
  • Gözüm S, Tezel A, Koc M (2003). Complementary alternative treatments used by patients with cancer in eastern Turkey. Cancer nursing 26(3): 230–236. Doi:10.1097/00002820-200306000-00010
  • Karakol P, Saraydin SU, Bozkurt M, Hepokur C, Inan ZDS, Turan M (2022). Anticancer Effects of Urtica Dioica in Breast Cancer. Asian Pacific Journal of Cancer Prevention, 23(2): 673–681. Doi:10.31557/APJCP.2022.23.2.673
  • Konrad L, Müller HH, Lenz C, Laubinger H, Aumüller G, Lichius JJ (2000). Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta Medica, 66(1): 44–47. Doi:10.1055/s-2000-11117
  • Körpe DA, Işeri ÖD, Sahin FI, Cabi E, Haberal M (2013). High-antibacterial activity of Urtica spp. seed extracts on food and plant pathogenic bacteria. International Journal of Food Sciences and Nutrition, 64(3): 355–362. Doi:10.3109/09637486.2012.734290
  • Levy A, Sivanesan D, Murugan R, Quinonez Y, Jaffe M, Rathinavelu A, Fattahi S, Ardekani AM, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, Akhavan-niaki H (2013). Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells : Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis. Asian Pacific Journal of Cancer Prevention, 13(5): 711–717
  • Mannila E, Marti-Quijal FJ, Selma-Royo M, Calatayud M, Falcó I, la Fuente B de, Barba FJ, Collado MC, Linderborg KM (2022). In Vitro Bioactivities of Food Grade Extracts from Yarrow (Achillea millefolium L.) and Stinging Nettle (Urtica dioica L.) Leaves. Plant Foods for Human Nutrition, 78: 132–138. Doi:10.1007/s11130-022-01020-y
  • Mansoori B, Mohammadi A, Hashemzadeh S, Shirjang S, Baradaran A, Asadi M, Doustvandi MA, Baradaran B (2017). Urtica dioica extract suppresses miR-21 and metastasis-related genes in breast cancer. Biomedicine & Pharmacotherapy, 93: 95–102. Doi:10.1016/j.biopha.2017.06.021
  • Mohammadi A, Mansoori B, Baradaran PC, Khaze V, Aghapour M, Farhadi M, Baradaran B (2017). Urtica dioica Extract Inhibits Proliferation and Induces Apoptosis and Related Gene Expression of Breast Cancer Cells In Vitro and In Vivo. Clinical Breast Cancer, 17(6): 463–470. Doi:10.1016/j.clbc.2017.04.008
  • Mohammadi A, Mansoori B, Goldar S, Shanehbandi D, Khaze V, Mohammadnejad L, Baghbani E, Baradaran B (2016). Effects of Urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-MB-468). Cellular and molecular biology (Noisy-le-Grand, France) 62(2): 62–67
  • Özkol H, Musa D, Tuluce Y, Koyuncu I (2012). Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug and Chemical Toxicology, 35(3): 251–257. Doi:10.3109/01480545.2011.598531
  • Paulauskienė A, Tarasevičienė Ž, Laukagalis V (2021). Influence of harvesting time on the chemical composition of wild stinging nettle (Urtica dioica l.). Plant,s 10(4). Doi:10.3390/plants10040686
  • Sayhan MB, Kanter M, Oguz S, Erboga M (2012). Protective effect of Urtica dioica L. on renal ischemia/reperfusion injury in rat. Journal of Molecular Histology, 43(6): 691–698. Doi:10.1007/s10735-012-9436-9
  • Shonte TT, Duodu KG, Kock HL de (2020). Effect of drying methods on chemical composition and antioxidant activity of underutilized stinging nettle leaves. Heliyon, 6(5). Doi:10.1016/j.heliyon.2020.e03938
  • Skalozubova TA, Reshetova VO (2013). Leaves of common nettle (Urtica dioica L.) as a source of ascorbic acid (vitamin C). World Applied Sciences Journal, 28(2): 250–253. Doi:10.5829/idosi.wasj.2013.28.02.13792
  • Upton R (2013). Stinging nettles leaf (Urtica dioica L.): Extraordinary vegetable medicine. Journal of Herbal Medicine, 3(1): 9–38. Doi:10.1016/j.hermed.2012.11.001
  • Veiga M, Costa EM, Silva S, Pintado M (2020). Impact of plant extracts upon human health: A review. Critical Reviews in Food Science and Nutrition, 60(5): 873–886. Doi:10.1080/10408398.2018.1540969

In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement

Yıl 2024, Cilt: 38 Sayı: 1, 140 - 146, 30.04.2024

Serol Korkmaz Hanife Banu Aydın İrem Korkmaz

Öz

Antioxidant capacity, cytotoxicity on two vital cell lines and the anti-cancer activity of methanolic Urtica dioica L. leaf extract (UDE) collected from Duzkoy, Giresun, Turkey were studied by determining safe concentration. The antioxidant capacity of the extract was expressed as vitamin C equivalency by spectrophotometric MTT assay. The cytotoxic concentration 50% was measured by the linearity between UDE concentrations (CC50) and the cell viability of non-cancer kidney cell lines (BHK-21, MDBK). The anti-cancer activity was conducted on human hepatocellular carcinoma cells (HepG2) by determining inhibition concentration (IC50) on cell proliferation. The vitamin C equivalence of UDE increased linearly by increasing the concentration. The cytotoxic and non-toxic concentrations of UDE were determined on BHK-21 and MDBK with 15.71 mg/ml and 5.14 mg/ml of CC50 respectively. The extract inhibited the proliferation of human hepatocellular carcinoma cells with a 2.46 mg/ml of IC50. In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract. The dose-response results showed that Urtica dioica L. leaf extract could have low cytotoxicity, but potential anti-cancer activity at safe concentrations.

Anahtar Kelimeler

anti-cancer cytotoxicity nettle Urtica dioica L.

Etik Beyan

Not required.

Destekleyen Kurum

This research received no external funding

Teşekkür

The authors thank Prof. Dr. Zafer Odabas, Prof. Dr. Hasan Kilic, and Prof. Dr. Aysen Gargili Keles (Head of the Basic Health Sciences Department and Research Laboratory, Marmara University). The extraction method used in this study is from a part of the MSc thesis of Hanife Banu Aydin supervised by B. Irem Omurtag Korkmaz.

Kaynakça

  • Alp E, Aksu MI (2010). Effects of water extract of Urtica dioica L. and modified atmosphere packaging on the shelf life of ground beef. Meat Science, 86(2): 468–473. Doi:10.1016/j.meatsci.2010.05.036
  • Aydin HB, Korkmaz S, Omurtag Korkmaz BI (2023). Antibacterial and antiviral activities of stinging nettle (Urtica dioica L.) leaf extract on norovirus and campylobacter jejuni as foodborne pathogens. Journal of Microbiology, Biotechnology and Food Sciences, 13(1):9768. Doi:10.55251/jmbfs.9768
  • Carvalho AR, Costa G, Figueirinha A, Liberal J, Prior JAV, Lopes MC, Cruz MT, Batista MT (2017). Urtica spp.: Phenolic composition, safety, antioxidant and anti-inflammatory activities. Food Research International, 99: 485–494. Doi:10.1016/j.foodres.2017.06.008
  • Dhouibi R, Affes H, Salem M Ben, Hammami S, Sahnoun Z, Zeghal KM, Ksouda K (2020). Screening of pharmacological uses of Urtica dioica and others benefits. Progress in Biophysics and Molecular Biology, 150: 67–77. Doi:10.1016/j.pbiomolbio.2019.05.008
  • Dini I (2019). An overview of functional beverages. Woodhead Publishing, MA, USA. p.1–40. Doi:10.1016/B978-0-12-816397-9.00001-7
  • Durak I, Biri H, Devrim E, Sözen S, Avcı A (2004). Aqueous extract of urtica dioica makes significant inhibition on adenosine deaminase activity in prostate tissue from patients with prostate cancer. Cancer Biology & Therapy, 3(9): 855–857. Doi:10.4161/cbt.3.9.1038
  • El-Saber Batiha G, Hussein DE, Algammal AM, George TT, Jeandet P, Al-Snafi AE, Tiwari A, Pagnossa JP, Lima CM, Thorat ND, Zahoor M, El-Esawi M, Dey A, Alghamdi S, Hetta HF, Cruz-Martins N (2021). Application of natural antimicrobials in food preservation: Recent views. Food Control, 126. Doi:10.1016/j.foodcont.2021.108066
  • Esposito S, Bianco A, Russo R, Maro A Di, Isernia C, Pedone PV (2019). Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer Treatment. Molecules, 24(15). Doi:10.3390/molecules24152753
  • European Commission (2023) Novel Food Catalogue. https://webgate.ec.europa.eu/fip/novel_food_catalogue/ (accessed date: 28.09.2023).
  • Fattahi S, Ardekani AM, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, Akhavan-Niaki H (2013). Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line. Asian Pacific Journal of Cancer Prevention, 14(9): 5317–5323
  • Fattahi S, Ghadami E, Asouri M, Ardekanid AM, Akhavan-Niaki H (2018). Urtica dioica inhibits cell growth and induces apoptosis by targeting Ornithine decarboxylase and Adenosine deaminase as key regulatory enzymes in adenosine and polyamines homeostasis in human breast cancer cell lines. Cellular and Molecular Biology, 64(3): 97–102
  • Flores-Ocelotl MR, Rosas-Murrieta NH, Moreno DA, Vallejo-Ruiz V, Reyes-Leyva J, Domínguez F, Santos-López G (2018). Taraxacum officinale and Urtica dioica extracts inhibit dengue virus serotype 2 replication in vitro. BMC complementary and alternative medicine, 18(1): 95. Doi:10.1186/s12906-018-2163-3
  • Garcìa LM, Ceccanti C, Negro C, Bellis L De, Incrocci L, Pardossi A, Guidi L (2021). Effect of drying methods on phenolic compounds and antioxidant activity of Urtica dioica L. leaves. Horticulturae, 7(1): 1–12. Doi:10.3390/horticulturae7010010
  • Gözüm S, Tezel A, Koc M (2003). Complementary alternative treatments used by patients with cancer in eastern Turkey. Cancer nursing 26(3): 230–236. Doi:10.1097/00002820-200306000-00010
  • Karakol P, Saraydin SU, Bozkurt M, Hepokur C, Inan ZDS, Turan M (2022). Anticancer Effects of Urtica Dioica in Breast Cancer. Asian Pacific Journal of Cancer Prevention, 23(2): 673–681. Doi:10.31557/APJCP.2022.23.2.673
  • Konrad L, Müller HH, Lenz C, Laubinger H, Aumüller G, Lichius JJ (2000). Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta Medica, 66(1): 44–47. Doi:10.1055/s-2000-11117
  • Körpe DA, Işeri ÖD, Sahin FI, Cabi E, Haberal M (2013). High-antibacterial activity of Urtica spp. seed extracts on food and plant pathogenic bacteria. International Journal of Food Sciences and Nutrition, 64(3): 355–362. Doi:10.3109/09637486.2012.734290
  • Levy A, Sivanesan D, Murugan R, Quinonez Y, Jaffe M, Rathinavelu A, Fattahi S, Ardekani AM, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, Akhavan-niaki H (2013). Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells : Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis. Asian Pacific Journal of Cancer Prevention, 13(5): 711–717
  • Mannila E, Marti-Quijal FJ, Selma-Royo M, Calatayud M, Falcó I, la Fuente B de, Barba FJ, Collado MC, Linderborg KM (2022). In Vitro Bioactivities of Food Grade Extracts from Yarrow (Achillea millefolium L.) and Stinging Nettle (Urtica dioica L.) Leaves. Plant Foods for Human Nutrition, 78: 132–138. Doi:10.1007/s11130-022-01020-y
  • Mansoori B, Mohammadi A, Hashemzadeh S, Shirjang S, Baradaran A, Asadi M, Doustvandi MA, Baradaran B (2017). Urtica dioica extract suppresses miR-21 and metastasis-related genes in breast cancer. Biomedicine & Pharmacotherapy, 93: 95–102. Doi:10.1016/j.biopha.2017.06.021
  • Mohammadi A, Mansoori B, Baradaran PC, Khaze V, Aghapour M, Farhadi M, Baradaran B (2017). Urtica dioica Extract Inhibits Proliferation and Induces Apoptosis and Related Gene Expression of Breast Cancer Cells In Vitro and In Vivo. Clinical Breast Cancer, 17(6): 463–470. Doi:10.1016/j.clbc.2017.04.008
  • Mohammadi A, Mansoori B, Goldar S, Shanehbandi D, Khaze V, Mohammadnejad L, Baghbani E, Baradaran B (2016). Effects of Urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-MB-468). Cellular and molecular biology (Noisy-le-Grand, France) 62(2): 62–67
  • Özkol H, Musa D, Tuluce Y, Koyuncu I (2012). Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug and Chemical Toxicology, 35(3): 251–257. Doi:10.3109/01480545.2011.598531
  • Paulauskienė A, Tarasevičienė Ž, Laukagalis V (2021). Influence of harvesting time on the chemical composition of wild stinging nettle (Urtica dioica l.). Plant,s 10(4). Doi:10.3390/plants10040686
  • Sayhan MB, Kanter M, Oguz S, Erboga M (2012). Protective effect of Urtica dioica L. on renal ischemia/reperfusion injury in rat. Journal of Molecular Histology, 43(6): 691–698. Doi:10.1007/s10735-012-9436-9
  • Shonte TT, Duodu KG, Kock HL de (2020). Effect of drying methods on chemical composition and antioxidant activity of underutilized stinging nettle leaves. Heliyon, 6(5). Doi:10.1016/j.heliyon.2020.e03938
  • Skalozubova TA, Reshetova VO (2013). Leaves of common nettle (Urtica dioica L.) as a source of ascorbic acid (vitamin C). World Applied Sciences Journal, 28(2): 250–253. Doi:10.5829/idosi.wasj.2013.28.02.13792
  • Upton R (2013). Stinging nettles leaf (Urtica dioica L.): Extraordinary vegetable medicine. Journal of Herbal Medicine, 3(1): 9–38. Doi:10.1016/j.hermed.2012.11.001
  • Veiga M, Costa EM, Silva S, Pintado M (2020). Impact of plant extracts upon human health: A review. Critical Reviews in Food Science and Nutrition, 60(5): 873–886. Doi:10.1080/10408398.2018.1540969
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tıbbi ve Aromatik Bitkiler
Bölüm Araştırma Makalesi
Yazarlar

Serol Korkmaz 0000-0001-8970-6883

Hanife Banu Aydın 0000-0002-4699-8828

İrem Korkmaz 0000-0001-7918-6212

Erken Görünüm Tarihi 30 Nisan 2024
Yayımlanma Tarihi 30 Nisan 2024
Gönderilme Tarihi 29 Eylül 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 38 Sayı: 1

Kaynak Göster

EndNote Korkmaz S, Aydın HB, Korkmaz İ (01 Nisan 2024) In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement. Selcuk Journal of Agriculture and Food Sciences 38 1 140–146.
 Kapak Resmi İndir

Selcuk Journal of Agriculture and Food Sciences Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.