MULTILAYER ANALYSIS OF NICOTINE-INDUCED GENE EXPRESSION ALTERATIONS IN BREAST CANCER CELLS USING CLUSTERING AND SUPERVISED LEARNING METHODS

Taybe Alabed; Sema Servi

doi:10.17780/ksujes.1730962

EN TR

MULTILAYER ANALYSIS OF NICOTINE-INDUCED GENE EXPRESSION ALTERATIONS IN BREAST CANCER CELLS USING CLUSTERING AND SUPERVISED LEARNING METHODS

Abstract

Nicotine is known not only for its addictive properties but also for its potential to alter the genetic structure of cancer cells. This study investigates the genetic alterations caused by chronic nicotine exposure in HCC38 breast cancer cells through a multi-layered computational analysis. Using K-means clustering and seven supervised machine learning algorithms, differentially expressed genes were grouped into three clusters and used as class labels in classification models. Logistic Regression achieved the highest performance with 98.76% accuracy and an F1 score of 0.9869. The Friedman test was applied to evaluate the statistical significance of performance differences among classifiers, and multi-class ROC curves were used to demonstrate their discriminative power. The findings indicate that nicotine exposure leads to genetic reprogramming and activates inflammation-related gene pathways in specific cellular subpopulations. These results highlight the utility of machine learning methods in uncovering biologically meaningful gene expression patterns in cancer research.

Keywords

References

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Details

Primary Language

English

Subjects

Artificial Intelligence (Other)

Journal Section

Research Article

Authors

Taybe Alabed
0009-0009-3722-2911
Türkiye

Sema Servi ^*
0000-0003-2069-9085
Türkiye

Publication Date

September 3, 2025

Submission Date

July 1, 2025

Acceptance Date

August 15, 2025

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Year 1970 Volume: 28 Number: 3

DOI

https://doi.org/10.17780/ksujes.1730962

IZ

https://izlik.org/JA49KM99AT

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RIS / Bibtex

APA

Alabed, T., & Servi, S. (2025). MULTILAYER ANALYSIS OF NICOTINE-INDUCED GENE EXPRESSION ALTERATIONS IN BREAST CANCER CELLS USING CLUSTERING AND SUPERVISED LEARNING METHODS. Kahramanmaraş Sütçü İmam Üniversitesi Mühendislik Bilimleri Dergisi, 28(3), 1558-1573. https://doi.org/10.17780/ksujes.1730962

MULTILAYER ANALYSIS OF NICOTINE-INDUCED GENE EXPRESSION ALTERATIONS IN BREAST CANCER CELLS USING CLUSTERING AND SUPERVISED LEARNING METHODS

MULTILAYER ANALYSIS OF NICOTINE-INDUCED GENE EXPRESSION ALTERATIONS IN BREAST CANCER CELLS USING CLUSTERING AND SUPERVISED LEARNING METHODS

Abstract

Keywords

KÜMELEME VE GÖZETİMLİ ÖĞRENME YÖNTEMLERİ KULLANILARAK MEME KANSERİ HÜCRELERİNDE NİKOTİNLE TETİKLENEN GEN EKSPRESYON DEĞİŞİKLİKLERİNİN ÇOK KATMANLI ANALİZİ

Öz

Anahtar Kelimeler

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