MİKROBİYAL NANOPARTİKÜL SENTEZİNDE YEREL LAKTİK ASİT BAKTERİ SUŞLARININ POTANSİYELİNİN BELİRLENMESİ

Özgül Yazar; Mehmet Tokatlı

doi:10.17780/ksujes.1736762

EN TR

DETERMINATION OF THE POTENTIAL OF LOCAL LACTIC ACID BACTERIA STRAINS IN MICROBIAL NANOPARTICLE SYNTHESIS

Abstract

This study aimed to determine the potential of lactic acid bacteria (LAB) strains for the microbial synthesis of titanium, iron, and silver metal nanoparticles using metal precursors such as K2TiF6, TiO2, Fe2O3, FeCl3, AgNO3, and dAgNO3. To achieve this, 141 LAB strains were initially screened for their growth in MRS-Agar media containing various concentrations of metal precursors (0.02-0.2M). This process identified 24 LAB strains exhibiting resistance to these metal precursors. Subsequently, a metal precursor solution (0.01M) was added to the culture media, including the cell extracts of these 24 LAB strains, and incubated at 30°C for 24 hours to facilitate NP synthesis. According to the spectrum scan results for NP synthesis by the 24 LAB strains that showed resistance and grew in metal-precursor-supplemented MRS-Agar media, absorbance values ranged from 4.5-34.1 for FeNPs, 2.2-39.1 for AgNPs, and 3.0-37.8 for TiNPs. These strains are predicted to possess a high capability for NP synthesis from metal precursors. The LAB strains that yielded the highest absorbance values in NP synthesis were, respectively: Lactiplantibacillus. namurensis MF112 for AgNPs (39.1 ABS), Pediococcus ethanolidurans MF115 for TiNPs (37.8 ABS), and Lactiplantibacillus namurensis MF112 for FeNPs (34.1 ABS).

Keywords

MİKROBİYAL NANOPARTİKÜL SENTEZİNDE YEREL LAKTİK ASİT BAKTERİ SUŞLARININ POTANSİYELİNİN BELİRLENMESİ

Öz

Bu çalışmada laktik asit bakteri (LAB) suşlarının K2TiF6 ve TiO2, Fe2O3, FeCl3, AgNO3 ve dAgNO3 metal öncüleri kullanılarak mikrobiyal titanyum, demir ve gümüş metal nanopartikül (NP) sentezleme potansiyelleri belirlenmiştir. Bunun için 141 LAB suşunun farklı konsantrasyonlarda (0.02-0.2M) metal öncü içeren MRS-Agar besiyerlerinde gelişimleri izlenerek metal öncülere direnç gösteren 24 LAB suşu belirlenmiştir. Daha sonra 24 LAB suşunun hücre ekstraktını da içeren kültür ortamlarına metal öncü çözeltisi (0.01M) ilave edilerek nanopartikül sentezi için 30˚C’de 24 saat boyunca inkübasyona bırakılmıştır. Metal öncü katkılı MRS-Agar besiyerinde direnç göstererek geliştiği belirlenen 24 LAB (Levilactobacillus brevis, Lactiplantibacillus plantarum, Pediococcus ethanolidurans, Lactobacillus buchneri, Pediococcus parvulus, Lactiplantibacillus namurensis, Lactiplantibacillus diolivoran, Lacticaseibacillus parabrevis) suşu ile NP sentezinin belirlenmesi için gerçekleştirilen spektrum tarama sonuçlarına göre; FeNP için 4.5-34.1; AgNP için 2.2-39.1 ve TiNP için 3.0-37.8 aralığında absorbans değerleri ölçülmüştür. NP sentezinde en yüksek absorbans değerlerinin ölçüldüğü LAB suşları sırasıyla; AgNP için L. namurensis MF112 (39.1 ABS), TiNP için P. ethanolidurans MF115 (37.8 ABS) ve FeNP için L. namurensis MF112 (34.1 ABS) olarak tespit edilmiştir. Bu suşların metal öncülerden NP sentezleme potansiyelinin olabileceği tespit edilmiştir. Çalışma sonucunda elde edilen veriler uygun LAB suşlarının kullanımı ile mikrobiyal NP üretimi optimizasyonu ve NP karakterizasyonu gibi daha ileri çalışmalara ışık tutacağı ön görülmektedir.

Anahtar Kelimeler

Supporting Institution

Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi Koordinatörlüğü (BAP: 2023/25)

Project Number

2023/25

Ethical Statement

Bu çalışmanın, özgün bir çalışma olduğunu; çalışmanın hazırlık, veri toplama, analiz ve bilgilerin sunumu olmak üzere tüm aşamalarından bilimsel etik ilke ve kurallarına uygun davrandığımı; bu çalışma kapsamında elde edilmeyen tüm veri ve bilgiler için kaynak gösterdiğimi ve bu kaynaklara kaynakçada yer verdiğimi; kullanılan verilerde herhangi bir değişiklik yapmadığımı, tüm şartlarını ve koşullarını kabul ederek etik görev ve sorumluluklara riayet ettiğimi beyan ederim.

Thanks

Bu çalışma Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi Koordinatörlüğü (BAP: 2023/25) tarafından finanse edilmiştir.

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Details

Primary Language

Turkish

Subjects

Food Engineering

Journal Section

Research Article

Authors

Özgül Yazar ^*
0000-0001-5154-3193
Türkiye

Mehmet Tokatlı
0000-0001-6264-9102
Türkiye

Publication Date

December 3, 2025

Submission Date

July 7, 2025

Acceptance Date

November 6, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.17780/ksujes.1736762

IZ

https://izlik.org/JA77JR87CS

Cite

RIS / Bibtex

APA

Yazar, Ö., & Tokatlı, M. (2025). MİKROBİYAL NANOPARTİKÜL SENTEZİNDE YEREL LAKTİK ASİT BAKTERİ SUŞLARININ POTANSİYELİNİN BELİRLENMESİ. Kahramanmaraş Sütçü İmam Üniversitesi Mühendislik Bilimleri Dergisi, 28(4), 1916-1927. https://doi.org/10.17780/ksujes.1736762