1. Kahramanmaraş Sütçü İmam Üniversitesi Mühendislik Bilimleri Dergisi
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Year 2017, Volume 20 , Issue 1, Pages 10 - 20 2017-04-27

KUZEY IRAK’TA ÜRETİLEN TAVUK ETLERİNDE SALMONELLA İZOLASYONU
ISOLATION OF SALMONELLA FROM CHICKEN MEATS IN NORTHERN IRAQ

Abdulrezzaq Haji Haji [1] , Ahmet İNANÇ [2]

Abstract

Makale Şablonu

Bu çalışmada, Duhok ve Erbil’deki lokal market ve kasaplardan temin edilen taze ve dondurulmuş tavuk etlerinde Salmonella yaygınlığı araştırılmıştır. Bu amaçla, 50 taze ve 50 dondurulmuş tavuk eti kültürel, biyokimyasal ve serolojik yöntemlerle incelenmiştir. Ayrıca, elde edilen Salmonella izolatlarının 13 farklı antibiyotiğe hassasiyetleri ölçülmüştür. Uygulan kültürel metotta, 100 örnekten 11’inde Salmonella kolonileri görülmüştür. Bunlardan 9 tanesinin taze (%18), 2 tanesinin de (%4) dondurulmuş tavuk etlerinden ya da illere göre, 7 tanesinin (%15.6) Erbil’den 4 tanesinin de Duhok’tan (%7.3) toplanan tavuk etlerinden ileri geldiği bulunmuştur. Salmonella şüphesi bulunan 11 kültür daha sonra biyokimyasal doğrulama testlere tabi tutulmuş ve geleneksel metotlarla yapılan testlerde 8’inin ve RapIDTM ONE sistemi ile yapılan testte ise 11’inin de Salmonella olduğu görülmüştür. Somatic (O) and flagellar (H) antiseralar ile yapılan serolojik testlerde, Salmonella şüphesi bulunan 11 kültür aglütinasyon-pozitif bulunmuştur. Irak Merkez Laboratuvarında yaptırılan serotip çalışmasında, 8 kültürde S. Typhimurium, 3 kültürde ise S. Anatum bulunmuştur. Her iki serotipinde 13 antibiyotikten sadece 4 tanesine (CT, C, CIP ve TE), ayrıca S. Anatum’un 2 ayrı antibiyotiğe daha duyarlı olduğu görülmüştür. 

Manuscript Template

The present study was conducted to isolate and confirm Salmonella in fresh and frozen chicken meats collected in local markets and butcheries in Duhok and Arbil, Northern Iraq. In this respect, fifty fresh and fifty frozen chicken meats were analyzed by the cultural, biochemical and serological methods. The suspected cultures were later subjected to the antibiotic resistance test using 13 known antibiotics. The cultural method for isolation of Salmonella indicated that 11 agar plates (9 from fresh (18%) and 2 from frozen (4%)) were probably suspected with Salmonella. Seven suspected meat samples was collected in Arbil (15.6%) and 4 in Duhok (7.3%). The biochemical confirmation tests performed later confirmed that 8 of 11 suspected culture contained Salmonella. The RapIDTM ONE test for further confirmation showed that all 11 suspected cultures probably infected with Salmonella with two different species. Using somatic (O) and flagellar (H) antisera, it was observed agglutination of the suspected cultures with polyvalent O and H antisera, indicating the suspected cultures contained Salmonella. The cultures were serotyped by the Central Health Laboratory, and confirmed that 8 of them were infected with S. Typhimurium, and 3 of them with S. Anatum. S. typhimurium and S. anatum were found to be sensitive only to CT, C, CIP and TE. S. anatum also exhibited sensitivity to K and TMP.


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Details

Subjects Engineering
Journal Section Research Articles
Authors

Author: Abdulrezzaq Haji Haji

Author: Ahmet İNANÇ
Country: Turkey
Dates

Application Date : January 31, 2017
Acceptance Date : June 4, 2020
Publication Date : April 27, 2017

Cite

APA Haji, A , İNANÇ, A . (2017). KUZEY IRAK’TA ÜRETİLEN TAVUK ETLERİNDE SALMONELLA İZOLASYONU. Kahramanmaraş Sütçü İmam Üniversitesi Mühendislik Bilimleri Dergisi , 20 (1) , 10-20 . DOI: 10.17780/ksujes.288798
Authors of the Article
Abdulrezzaq Haji Haji [1]
Ahmet İNANÇ [2]
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